Endonuclease Activity of Phenol Oxidase From Musca domestica Larvae
Endonuclease Activity of Phenol Oxidase From Musca domestica Larvae
复制标题
DOI：
10.2307/25470688
复制
发表时间：
2008-08
期刊：
影响因子：
--
通讯作者：
Shaoguang Sun;Weiquan Liu;Jigui Wang;Shu-Yan Yang;Ling Gu;Yan Hong;Dan-Dan Shang-Dan;B. Wang;Xiaoming Su;S. Qi
中科院分区：
文献类型：
--
作者：
Shaoguang Sun;Weiquan Liu;Jigui Wang;Shu-Yan Yang;Ling Gu;Yan Hong;Dan-Dan Shang-Dan;B. Wang;Xiaoming Su;S. Qi
酚氧化酶（PO）是一种具有加氧酶活性的含铜酶，可以将单酚或二酚转化为醌，在节肢动物的黑化反应中发挥重要作用。本文报道了家蝇幼虫PO的一个新特性：具有耐热性内切酶活性，即使在80° C下加热20 min，PO仍能降解质粒DNA。表达产物与纯化的PO具有相同的性质。我们的数据表明PO是一种双功能酶，同时表现出加氧酶和核酸内切酶活性，这表明这种重要分子在M的先天反应中发挥了新的作用。南极洲
Phenol oxidase (PO), a copper-containing enzyme with oxygenase activity, can convert mono- or diphenol into quinone and plays an important role in the arthropod melanization reaction. Here, we report a new property of PO from Musca domestica larvae: a thermotolerant endonuclease activity, by which PO can degrade plasmid DNA even after being heated to 80° C for 20 min. We cloned PO cDNA, constructed the expression vector pVAX1-PO, and expressed it in HeLa cells. The expression product showed the same properties as purified PO. Our data indicate that PO is a bifunctional enzyme, exhibiting both oxygenase and endonuclease activity, suggesting new roles for this important molecule in the innate responses of M. domestica.