Two vaccine toxicity-related genes Agp and Hpx could prove useful for pertussis vaccine safety control
Two vaccine toxicity-related genes Agp and Hpx could prove useful for pertussis vaccine safety control
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DOI：
10.1016/j.vaccine.2006.12.059
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发表时间：
2007-04-30
期刊：
影响因子：
5.5
通讯作者：
Yamaguchi, Kazunari
中科院分区：
文献类型：
--
作者：
Hamaguchi, Isao;Imai, Jun-ichi;Yamaguchi, Kazunari
传统的动物试验，如白细胞促进试验，几十年来一直被用来评估百日咳疫苗的毒性。在这里，我们使用DNA微阵列检查了与疫苗毒性相关的基因表达。将传统的动物试验数据与基于DNA微阵列的基因表达数据进行比较，发现了一种与动物白细胞增多症高度相关的基因表达模式。在分析的10,490个大鼠基因中，发现两个基因，α1-酸性糖蛋白(AGP)和血凝素(HPX)，被毒素以剂量依赖的方式上调(通过基于微阵列的定量PCR进行检测)。在实验动物中，基因表达的变化非常小，结果具有很高的重复性。这些发现表明，疫苗处理动物的基因表达分析可以作为一种准确而简便的百日咳疫苗安全性评估方法。(C)2007爱思唯尔有限公司。保留所有权利。
Conventional animal tests such as leukocytosis promoting tests have been used for decades to evaluate toxicity of pertussis vaccine. Here, we examined gene expression in relation to the vaccine toxicity using a DNA microarray. Comparison of conventional animal test data with the DNA microarray-based gene expression data revealed a gene expression pattern highly correlated with leukocytosis in animals. Of 10,490 rat genes analyzed, two genes, alpha 1-acid-glycoprotein (Agp) and hemopexin (Hpx), were found up-regulated by the toxin administration in a dose-dependent manner (assayed by a quantitative PCR based on the microarray). Variation of the gene expression was very small amongst the test animals, and the results were highly reproducible. These findings suggest that gene expression analysis of vaccine-treated animals can be used as an accurate and simple method of pertussis vaccine safety assessment. (c) 2007 Elsevier Ltd. All rights reserved.