Recent advances in DNA sensors
Recent advances in DNA sensors
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DOI：
10.1039/b803083a
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发表时间：
2008-01-01
期刊：
影响因子：
4.2
通讯作者：
Mailley, Pascal
中科院分区：
文献类型：
--
作者：
Cosnier, Serge;Mailley, Pascal
DNA生物传感器的概念是由对用于基因检测的快速和高灵敏分析工具的需求所支撑的。它们的实施基于三个关键步骤：(1)将单链寡核苷酸探针固定在底物上；(2)杂交和(3)阅读。这些步骤涉及不同学科领域的互补知识，如表面物理和化学、分子电化学、微技术、光学、电子学和生物化学。我们在这里以非详尽的方式介绍了固定和检测这两个步骤的最新进展，这两个步骤依赖于阅读点数量或/和阅读策略的日益整合。
The concept of DNA biosensors is sustained by the need for rapid and highly sensitive analytical tools for genetic detection. Their implementation is based on three key steps: (i) immobilization of single-stranded oligonucleotide probes onto a substrate; (ii) hybridization and (iii) reading. These steps involve complementary knowledge in various disciplinary fields such as surface physics and chemistry, molecular electrochemistry, micro-technologies, optics, electronics and biochemistry. We present here, in a non-exhaustive way, the recent advances in the two steps of immobilization and detection that rely upon increasing integration of the number of reading points or/and of the reading strategy.