Construction of a cytogenetic dose-response curve for low-dose range gamma-irradiation in human peripheral blood lymphocytes using three-color FISH
Construction of a cytogenetic dose-response curve for low-dose range gamma-irradiation in human peripheral blood lymphocytes using three-color FISH
复制标题
DOI：
10.1016/j.mrgentox.2015.10.002
复制
发表时间：
2015-12-01
影响因子：
1.9
通讯作者：
Hirai, Momoki
中科院分区：
文献类型：
--
作者：
Suto, Yumiko;Akiyama, Miho;Hirai, Momoki
为了估算低剂量电离辐射照射后的生物剂量，采用三种不同颜色的染色体涂染探针进行荧光原位杂交(FISH)检测交换型染色体畸变率。使用一名女性捐献者的血液样本构建了参考剂量反应曲线，该女性捐赠者的淋巴细胞在常规培养条件下第二次有丝分裂时始终显示低频率的细胞。研究了从7个剂量点(0、50、100、150、200、250和300mGY)获得的总共约15.5万个中期的像差产额。用市售的1、2和4号染色体涂染探针进行原位杂交。借助自动图像捕获方法，可以相当准确和快速地检测到涉及涂抹的染色体的交换型畸变。7个剂量点的交换型像差(双着丝粒+易位)符合线性二次模型(y=0.0023+0.0015x+0.0819x(2)，P=0.83)。盲法试验证明了参考剂量-反应关系的重复性。在用另一献血者的血液样本进行的对照实验中，根据目前的参考曲线计算的估计剂量被证明与实际应用的物理剂量非常一致。目前的剂量-反应曲线可以作为评估细胞遗传学放射敏感性的个体差异的一种手段。(C)2015年提交人。爱思唯尔出版公司(Elsevier B.V.)
In order to estimate biological doses after low-dose ionizing radiation exposure, fluorescence in situ hybridization (FISH) using three differentially colored chromosome painting probes was employed to detect exchange-type chromosome aberrations. A reference dose response curve was constructed using blood samples from a female donor whose lymphocytes consistently exhibited a low frequency of cells at the second mitosis under routine culture conditions. Aberration yields were studied for a total of about 155 thousand metaphases obtained from seven dose-points of gamma irradiations (0, 50, 100, 150, 200, 250 and 300 mGy). In situ hybridization was performed using commercially available painting probes for chromosomes 1, 2 and 4. With the aid of an automated image-capturing method, exchange-type aberrations involving painted chromosomes were detected with considerable accuracy and speed. The results on the exchange-type aberrations (dicentrics plus translocations) at the seven dose-points showed a good fit to the linear-quadratic model (y=0.0023 + 0.0015x+ 0.0819x(2), P = 0.83). A blind test proved the reproducibility of the reference dose-response relationship. In the control experiments using blood samples from another donor, the estimated doses calculated on the basis of the present reference curve were proved to be in good agreement with the actual physical doses applied. The present dose-response curve may serve as a means to assess the individual differences in cytogenetical radio-sensitivities. (C) 2015 The Authors. Published by Elsevier B.V.