Galleria mellonella is an effective model to study Actinobacillus pleuropneumoniae infection.
Galleria mellonella is an effective model to study Actinobacillus pleuropneumoniae infection.
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DOI：
10.1099/mic.0.083923-0
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发表时间：
2015-02
期刊：
影响因子：
1.5
通讯作者：
M. F. Pereira;C. C. Rossi-C.;M. V. de Queiroz;G. Martins;C. Isaac;J. Bossé;Yanwen Li;B. Wren;V. Terra;Jon Cuccui;P. Langford;D. Bazzolli
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文献类型：
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作者：
M. F. Pereira;C. C. Rossi-C.;M. V. de Queiroz;G. Martins;C. Isaac;J. Bossé;Yanwen Li;B. Wren;V. Terra;Jon Cuccui;P. Langford;D. Bazzolli
胸膜肺炎放线杆菌是引起猪胸膜肺炎的病原菌，是一种严重危害人类健康的呼吸道疾病。其毒力取决于许多因素，如荚膜多糖、RTX毒素和铁获取系统。毒力分析可能需要易于使用的模型，近似哺乳动物感染，避免伦理问题。在这里，我们调查的潜在用途的蜡蛾大蜡螟作为一个信息模型的A。胸膜肺炎感染基因型不同的A.胸膜肺炎临床分离株能够在37 °C下杀死幼虫，但具有不同的LD 50值，范围为10（4）至10（7）c.f.u.每只幼虫。毒力最强的分离物（1022）能够在昆虫体内持续存在和复制，而毒力最低的分离物（780）则被迅速清除。我们观察到分离株1022感染后血细胞浓度、聚集和DNA损伤减少。感染G. Mellonella，表明幼虫背侧血管附近有强烈的细胞和体液免疫反应。在猪中发现，A。胸膜肺炎hfq突变体对G.梅洛内拉模型。此外，该模型可用于评估几种抗菌药物对A.体内胸膜肺炎。G.大蜡螟是一种廉价的替代感染模型，可用于研究A.胸膜肺炎，以及评估抗微生物剂对这种病原体的有效性。
Actinobacillus pleuropneumoniae is responsible for swine pleuropneumonia, a respiratory disease that causes significant global economic loss. Its virulence depends on many factors, such as capsular polysaccharides, RTX toxins and iron-acquisition systems. Analysis of virulence may require easy-to-use models that approximate mammalian infection and avoid ethical issues. Here, we investigate the potential use of the wax moth Galleria mellonella as an informative model for A. pleuropneumoniae infection. Genotypically distinct A. pleuropneumoniae clinical isolates were able to kill larvae at 37 °C but had different LD50 values, ranging from 10(4) to 10(7) c.f.u. per larva. The most virulent isolate (1022) was able to persist and replicate within the insect, while the least virulent (780) was rapidly cleared. We observed a decrease in haemocyte concentration, aggregation and DNA damage post-infection with isolate 1022. Melanization points around bacterial cells were observed in the fat body and pericardial tissues of infected G. mellonella, indicating vigorous cell and humoral immune responses close to the larval dorsal vessel. As found in pigs, an A. pleuropneumoniae hfq mutant was significantly attenuated for infection in the G. mellonella model. Additionally, the model could be used to assess the effectiveness of several antimicrobial agents against A. pleuropneumoniae in vivo. G. mellonella is a suitable inexpensive alternative infection model that can be used to study the virulence of A. pleuropneumoniae, as well as assess the effectiveness of antimicrobial agents against this pathogen.