Scleraxis lineage cells contribute to organized bridging tissue during tendon healing and identify a subpopulation of resident tendon cells
Scleraxis lineage cells contribute to organized bridging tissue during tendon healing and identify a subpopulation of resident tendon cells
复制标题
DOI：
10.1096/fj.201900130rr
复制
发表时间：
2019-07-01
期刊：
影响因子：
4.8
通讯作者：
Loiselle, Alayna E.
中科院分区：
文献类型：
--
作者：
Best, Katherine T.;Loiselle, Alayna E.
在肌腱愈合过程中，假设肌腱细胞驱动组织再生，而外源性细胞驱动病理性瘢痕形成。肌腱细胞通常被描述为一个同质的，成纤维细胞群体是阳性的标志物Scleraxis（Scx）。在成人肌腱愈合过程中，肌腱细胞是否定位于形成的瘢痕组织内存在争议。我们之前已经证明，S100钙结合蛋白A4（S100a4）是肌腱瘢痕形成的驱动因素，并标记了肌腱细胞的一个子集。Scx和S100a4之间的关系尚未被探索。在这项研究中，我们评估了成年小鼠屈肌腱修复后Scx谱系细胞（Scx（Lin））的定位，并在稳态和愈合过程中建立了Scx和S100a4之间的关系。我们发现成年Scx（Lin）位于疤痕组织内，并在肌腱愈合期间组织成细胞桥。此外，我们证明，标记Scx和S100a4标签不同的人群在肌腱的稳态和愈合过程中，与Scx发现在有组织的桥接组织和S100a4定位在整个疤痕区域。这些研究定义了一个异质性肌腱细胞环境，并证明了愈合过程中亚群的离散贡献。这些数据增强了我们对肌腱细胞环境的理解和能力。最好的，K。T.，Loiselle，A. E.巩膜轴谱系细胞有助于组织化的桥接组织在肌腱愈合和确定一个亚群的常驻肌腱细胞。
During tendon healing, it is postulated that tendon cells drive tissue regeneration, whereas extrinsic cells drive pathologic scar formation. Tendon cells are frequently described as a homogenous, fibroblast population that is positive for the marker Scleraxis (Scx). It is controversial whether tendon cells localize within the forming scar tissue during adult tendon healing. We have previously demonstrated that S100 calcium-binding protein A4 (S100a4) is a driver of tendon scar formation and marks a subset of tendon cells. The relationship between Scx and S100a4 has not been explored. In this study, we assessed the localization of Scx lineage cells (Scx(Lin)) following adult murine flexor tendon repair and established the relationship between Scx and S100a4 throughout both homeostasis and healing. We showed that adult Scx(Lin) localize within the scar tissue and organize into a cellular bridge during tendon healing. Additionally, we demonstrate that markers Scx and S100a4 label distinct populations in tendon during homeostasis and healing, with Scx found in the organized bridging tissue and S100a4 localized throughout the entire scar region. These studies define a heterogeneous tendon cell environment and demonstrate discrete contributions of subpopulations during healing. These data enhance our understanding and ability to target the cellular environment of the tendon.-Best, K. T., Loiselle, A. E. Scleraxis lineage cells contribute to organized bridging tissue during tendon healing and identify a subpopulation of resident tendon cells.