Moxibustion regulates inflammatory mediators and colonic mucosal barrier in ulcerative colitis rats
Moxibustion regulates inflammatory mediators and colonic mucosal barrier in ulcerative colitis rats
复制标题
DOI：
10.3748/wjg.v22.i8.2566
复制
发表时间：
2016-02-28
影响因子：
4.3
通讯作者：
Yan, Hong-Chi
中科院分区：
文献类型：
--
作者：
Ma, Tie-Ming;Xu, Na;Yan, Hong-Chi
目的：目的：观察麦粒灸不同穴位对溃疡性结肠炎（UC）大鼠模型的疗效及作用机制。将SD大鼠随机分为对照组、UC模型组、单穴米粒灸组、双穴米粒灸组艾灸组（CV 12、CV 4）、麦粒灸三穴组（CV 12、CV 4、ST 36）和药物组（n = 8/组）。采用三硝基苯磺酸灌肠法建立大鼠UC模型。直接灸治疗，1次/d，连续7 d。治疗前后进行疾病活动指数（DAI）评价。光镜下观察肠组织形态学变化。Western blot检测结肠组织中肿瘤坏死因子（TNF）-α和p38丝裂原活化蛋白激酶（p38 MAPK）的表达，逆转录PCR检测结肠组织中闭合蛋白（occludin）和闭合小带-1（ZO-1）mRNA的表达。与对照组相比，模型组肠黏膜不完整，腺体结构不规则，黏膜下水肿，充血，并有炎性细胞浸润。与对照组相比，模型组DAI评分、TNF-α和p38 MAPK表达均显著升高（P < 0.01），而occludin和ZO-1 mRNA表达均显著降低（P < 0.01）。与模型组相比，各治疗组结肠黏膜完整，腺体排列整齐。艾灸组DAI评分、TNF-α和p38 MAPK表达较对照组明显降低（P < 0.01），而occludin和ZO-1 mRNA表达较对照组明显升高（P < 0.01）。结论：麦粒灸可降低结肠组织中TNF-α和p38 MAPK的表达，增加occludin和ZO-1的表达，这可能是麦粒灸促进肠黏膜组织修复的机制之一。
AIM: To observe the efficacy and mechanism of grain-sized moxibustion at different acupoints in a rat model of ulcerative colitis (UC).METHODS: Sprague-Dawley rats were randomly divided into control, UC model, grain-sized moxibustion at a single acupoint (CV 12), grain-sized moxibustion at two acupoints (CV 12 and CV 4), grain-sized moxibustion at three acupoints (CV 12, CV 4, and ST 36), and medication groups (n = 8/group). The UC model was established by enema of trinitrobenzene sulfonic acid. Direct moxibustion was used once a day for 7 d. Disease activity index (DAI) was evaluated before and after the treatment. Morphologic changes of intestinal tissue were observed under an optical microscope. The expression of tumor necrosis factor (TNF)-alpha and p38 mitogen-activated protein kinase (p38MAPK) in colonic tissue was detected using Western blot, and the levels of occludin and zonula occludens-1 (ZO-1) mRNAs were detected using reverse transcription PCR.RESULTS: Compared with the control group, the intestinal mucosae were incomplete in the model group, glandular structures were irregular, and submucosae were edematous, hyperemic, and infiltrated with inflammatory cells. The DAI scores and expression of TNF-alpha and p38MAPK were increased significantly in the model group compared to controls (Ps < 0.01), while the mRNA levels of occludin and ZO-1 were reduced significantly (Ps < 0.01). Compared with the model group, colonic mucosa and the arrangement of glands were complete and regular in the treatment groups. DAI scores and the expression of TNF-alpha and p38MAPK were reduced significantly in moxibustion groups compared to controls (Ps < 0.01), while the mRNA levels of occludin and ZO-1 were increased significantly (Ps < 0.01). The improvements in the above indices in the three acupoints group and the medication group were superior to those in the single and two acupoints groups (all P < 0.05).CONCLUSION: Reduction of TNF-alpha and p38MAPK and increased expression of occludin and ZO-1 in colonic tissue represent a potential mechanism for improved intestinal mucosal tissue repair with grain-sized moxibustion.