Using Gold Nanoclusters As Selective Luminescent Probes for Phosphate-Containing Metabolites
Using Gold Nanoclusters As Selective Luminescent Probes for Phosphate-Containing Metabolites
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DOI：
10.1021/ac300332t
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发表时间：
2012-07-03
影响因子：
7.4
通讯作者：
Chen, Yu-Chie
中科院分区：
文献类型：
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作者：
Li, Po-Han;Lin, Ju-Yu;Chen, Yu-Chie
谷胱甘肽结合的金纳米团簇（AuNCs@GSH）在紫外光照射下能发出微红色的光致发光。当与铁离子螯合时，AuNCs@GSH的发光被淬灭（AuNCs@ GSH-Fe 3+），推测是由于纳米团簇和铁离子之间的有效电子转移。然而，我们发现，金纳米团簇的发光可以在含磷酸盐的分子的存在下恢复，这表明使用AuNCs@ GSH-Fe 3+复合物作为含磷酸盐的代谢物的选择性发光开关的可能性。含磷酸盐代谢物如腺苷-5 '-三磷酸（ATP）和焦磷酸盐在生物系统中起着重要作用。在这项研究中，我们证明了当与ATP和焦磷酸盐混合时，AuNCs@ GSH-Fe 3+的发光被打开，这可以很容易地通过肉眼观察到。这是由于磷酸盐和铁离子之间的高形成常数。当使用荧光光谱作为检测工具时，可以对含磷酸盐的代谢物如ATP和焦磷酸盐进行定量分析。ATP和焦磷酸盐的线性范围为50 μ M至亚毫摩尔，而ATP和焦磷酸盐的检测限分别为43 μ M和28 μ M。此外，我们证明了AuNCs@ GSH-Fe 3+的发光也可以在来自细胞裂解物和血浆的含磷酸盐代谢物的存在下打开。
Glutathione-bound gold nanoclusters (AuNCs@GSH) can emit reddish photoluminescence under illumination of ultraviolet light. The luminescence of the AuNCs@GSH is quenched when chelating with iron ions (AuNCs@GSH-Fe3+), presumably resulting from the effective electron transfer between the nanoclusters and iron ions. Nevertheless, we found that the luminescence of the gold nanoclusters can be restored in the presence of phosphate-containing molecules, which suggested the possibility of using AuNCs@GSH-Fe3+ complexes as the selective luminescent switches for phosphate-containing metabolites. Phosphate-containing metabolites such as adenosine-5'-triphosphate (ATP) and pyrophosphate play an important role in biological systems. In this study, we demonstrated that the luminescence of the AuNCs@GSH-Fe3+ is switched-on when mixing with ATP and pyrophosphate, which can readily be observed by the naked eye. It results from the high formation constants between phosphates and iron ions. When employing fluorescence spectroscopy as the detection tool, quantitative analysis for phosphate-containing metabolites such as ATP and pyrophosphate can be conducted. The linear range for ATP and pyrophosphate is 50 mu M to sub-millimolar, while the limit of detection for ATP and pyrophosphate are similar to 43 and similar to 28 mu M, respectively. Additionally, we demonstrated that the luminescence of the AuNCs@GSH-Fe3+ can also be turned on in the presence of phosphate-containing metabolites from cell lysates and blood plasma.