Stimuli-Responsive Cucurbit[7] uril-Mediated BSA Nanoassembly for Uptake and Release of Doxorubicin
Stimuli-Responsive Cucurbit[7] uril-Mediated BSA Nanoassembly for Uptake and Release of Doxorubicin
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DOI：
10.1002/asia.201601411
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发表时间：
2017-01-01
影响因子：
4.1
通讯作者：
Mohanty, Jyotirmayee
中科院分区：
文献类型：
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作者：
Barooah, Nilotpal;Kunwar, Amit;Mohanty, Jyotirmayee
我们报道了牛血清白蛋白（BSA）蛋白和葫芦[7]脲大环的无毒纳米组装体的构建以及其与金刚烷胺或pH的刺激响应性断裂，其恢复蛋白质结构和识别特性。组装显示出标准药物多柔比星（DOX）的有效装载和受控释放，并且在活细胞中验证了这一点。细胞活力研究证明，装载DOX的组件掩盖了DOX的细胞毒性，并且毒性可以根据需要在靶标处恢复，从而触发其治疗活化。这在癌细胞中更有效。此外，这种宿主辅助的蛋白质组装体也非常有希望用于稳定/保护天然蛋白质结构，这是防止/抑制蛋白质错误折叠和聚集的可行方法。
We report the construction of a non-toxic nano-assembly of bovine serum albumin (BSA) protein and the cucurbit[7] uril macrocycle as well as its stimuli-responsive breakage with adamantylamine or pH, which restores the protein structure and recognition properties. The assembly showed efficient loading and controlled release of a standard drug, doxorubicin (DOX), and the same was validated in live cells. The cell viability studies documented that the DOX-loaded assembly mask the cytotoxicity of DOX and the toxicity can be revived at the target on demand, triggering its therapeutic activation. This is found to be more effective in the cancer cells. In addition, such host-assisted protein assemblies are also highly promising for stabilizing/protecting the native protein structure, a viable approach to prevent/inhibit protein misfolding and aggregation.