Vomifoliol 9-O-α-arabinofuranosyl (1→6)-β-D-glucopyranoside from the leaves of Diospyros Kaki stimulates the glucose uptake in HepG2 and 3T3-L1 cells
Vomifoliol 9-O-α-arabinofuranosyl (1→6)-β-D-glucopyranoside from the leaves of Diospyros Kaki stimulates the glucose uptake in HepG2 and 3T3-L1 cells
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DOI：
10.1016/j.carres.2011.04.021
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发表时间：
2011-07-15
影响因子：
3.1
通讯作者：
Wang, Myeong-Hyeon
中科院分区：
文献类型：
--
作者：
Wang, Lan;Xu, Ming Lu;Wang, Myeong-Hyeon
首次从柿叶中分离得到一种新的α-葡萄糖苷酶抑制剂--vomifoliol 9-O-alpha-arabinofuranosyl（1 -> 6）-beta-D-glucopyranoside，并对其生物活性进行了研究。该抑制剂表现出强的抗α-葡萄糖苷酶活性，IC 50值为170.62 nM，并刺激HepG 2细胞中荧光D-葡萄糖类似物2-[N-（7-硝基苯-2-氧杂-1，3-二唑-4-基）氨基]-2-脱氧-D-葡萄糖（2-NBDG）的摄取以高于胰岛素对照的速率剂量依赖性增加。还发现其与脂肪细胞分化和3 T3-L1细胞对2-NBDG摄取的适度增加相关。这些发现表明，番木鳖酚9-O-α-阿拉伯呋喃糖基（1 -> 6）-β-D-吡喃葡萄糖苷可以增加外周葡萄糖作为胰岛素增敏剂对抗2型糖尿病。皇冠版权所有（C）2011由爱思唯尔有限公司出版。保留所有权利。
A novel alpha-glucosidase inhibitor, vomifoliol 9-O-alpha-arabinofuranosyl (1 -> 6)-beta-D-glucopyranoside, was isolated for the first time from leaves of Diospyros Kaki and its bioactivity analyzed. This inhibitor exhibited strong anti-alpha-glucosidase activity with an IC50 value of 170.62 nM and stimulated a dose-dependent increase in the uptake of a fluorescent D-glucose analog, 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino]-2-deoxy-D-glucose (2-NBDG), in HepG2 cells at a rate higher than that of insulin controls. It was also found to be associated with adipocyte differentiation and moderate increases in 2-NBDG uptake by 3T3-L1 cells. These findings suggest that vomifoliol 9-O-a-arabinofuranosyl (1 -> 6)-beta-D-glucopyranoside could augment peripheral glucose as an insulin-sensitizing agent against Type 2 diabetes mellitus. Crown Copyright (C) 2011 Published by Elsevier Ltd. All rights reserved.