The FgNot3 Subunit of the Ccr4-Not Complex Regulates Vegetative Growth, Sporulation, and Virulence in Fusarium graminearum.
The FgNot3 Subunit of the Ccr4-Not Complex Regulates Vegetative Growth, Sporulation, and Virulence in Fusarium graminearum.
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DOI：
10.1371/journal.pone.0147481
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Lee YW
中科院分区：
文献类型：
--
作者：
Bui DC;Son H;Shin JY;Kim JC;Kim H;Choi GJ;Lee YW
Ccr4-Not 复合体在进化上是保守的，对于真核细胞的多种细胞功能很重要。在本研究中，研究了该复合物的 FgNot3 亚基在植物病原真菌禾谷镰刀菌中的生物学作用。 FgNOT3 的缺失导致营养生长迟缓、孢子萌发迟缓、菌丝肿胀和过度分枝。 ΔFgnot3突变体还表现出有性和无性孢子形成受损、毒力降低以及与分生孢子发生相关的基因表达减少。 Fgnot3 缺失突变体对热应激敏感，而已知其他模型真核生物中的 NOT3 直向同源物是细胞壁完整性所必需的。我们发现 FgNot3 作为次生代谢产物（包括单端孢菌烯和玉米赤霉烯酮）产生的负调节因子。 Ccr4-Not 复合物的 Not 模块的其他组件的进一步功能表征表明该模块是保守的。在禾谷镰刀菌中，每个亚基主要在复合体的背景下发挥作用，并且在复合体之外可能具有不同的作用。这是第一项对丝状真菌中 Not 模块进行功能表征的研究，并为真菌发育中的信号转导途径提供了新的见解。
The Ccr4-Not complex is evolutionarily conserved and important for multiple cellular functions in eukaryotic cells. In this study, the biological roles of the FgNot3 subunit of this complex were investigated in the plant pathogenic fungus Fusarium graminearum. Deletion of FgNOT3 resulted in retarded vegetative growth, retarded spore germination, swollen hyphae, and hyper-branching. The ΔFgnot3 mutants also showed impaired sexual and asexual sporulation, decreased virulence, and reduced expression of genes related to conidiogenesis. Fgnot3 deletion mutants were sensitive to thermal stress, whereas NOT3 orthologs in other model eukaryotes are known to be required for cell wall integrity. We found that FgNot3 functions as a negative regulator of the production of secondary metabolites, including trichothecenes and zearalenone. Further functional characterization of other components of the Not module of the Ccr4-Not complex demonstrated that the module is conserved. Each subunit primarily functions within the context of a complex and might have distinct roles outside of the complex in F. graminearum. This is the first study to functionally characterize the Not module in filamentous fungi and provides novel insights into signal transduction pathways in fungal development.