Atrial natriuretic peptides inhibit conductive sodium uptake by rabbit inner medullary collecting duct cells.
Atrial natriuretic peptides inhibit conductive sodium uptake by rabbit inner medullary collecting duct cells.
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心钠素抑制兔内髓集合管细胞传导钠的摄取。
DOI：
10.1172/jci113663
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Brenner,BM
中科院分区：
文献类型：
--
作者：
Zeidel,ML;Kikeri,D;Silva,P;Burrowes,M;Brenner,BM
内髓集合管（IMCD）在容量调节激素（包括心房钠尿肽（ANP））的控制下影响净钠重吸收。这些研究探讨了IMCD细胞新鲜悬浮液中钠转运及其由ANP调节的机制。阿米洛利可抑制钠摄取，但对呋塞米、布美他尼和氢氯噻嗪不敏感。这些结果与钠通道或Na +/H+交换介导的摄取一致。为了确定钠通道的作用，通过在高钾培养基中预孵育，然后稀释到无钾培养基中来使细胞超极化。使用花青染料Di（S）-C3 - 5的膜电位测量证实了使用该方案的IMCD细胞的显著超极化。超极化使钠摄取的表观初始速率增加了4倍。阿米洛利和心钠素抑制电位刺激的钠摄取分别为73%和65%，这两种药物加在一起是不加和的。向超极化细胞中加入5 mM钠导致显著的阿米洛利敏感性去极化。在3 × 10（-7）M阿米洛利和5 × 10（-11）M ANP时，对电位驱动的钠摄取产生半数最大抑制。我们的结论是，钠进入IMCD细胞通过一个导电的，阿米洛利敏感的钠通道，这是由ANP调节。ANP抑制IMCD的腔钠进入似乎有助于这种激素在体内的显着利钠作用。
The inner medullary collecting duct (IMCD) effects net sodium reabsorption under the control of volume regulatory hormones, including atrial natriuretic peptides (ANP). These studies examined the mechanisms of sodium transport and its regulation by ANP in fresh suspensions of IMCD cells. Sodium uptake was inhibited by amiloride but insensitive to furosemide, bu-metanide, and hydrochlorthiazide. These results are consistent with uptake mediated by a sodium channel or Na+/H+ exchange. To determine the role of sodium channels, cells were hyperpolarized by preincubation in high potassium medium followed by dilution into potassium-free medium. Membrane potential measurements using the cyanine dye, Di(S)-C3-5 verified a striking hyperpolarization of IMCD cells using this protocol. Hyperpolarization increased the apparent initial rate of sodium uptake fourfold. Amiloride and ANP inhibited potential-stimulated sodium uptake 73% and 65%, respectively; the two agents together were not additive. Addition of 5 mM sodium to hyperpolarized cells resulted in a significant amiloride-sensitive depolarization. Half-maximal inhibition of potential-driven sodium uptake occurred at 3 X 10(-7) M amiloride, and 5 X 10(-11) M ANP. We conclude that sodium enters IMCD cells via a conductive, amiloride-sensitive sodium channel, which is regulated by ANP. ANP inhibition of luminal sodium entry in the IMCD appears to contribute to the marked natriuretic effect of this hormone in vivo.
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兔肾髓质集合管细胞的细胞内pH调节。
DOI：
10.1172/jci112486
发表时间：
1986
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Zeidel,ML;Silva,P;Seifter,JL
通讯作者：
Seifter,JL
DOI：
10.1152/ajprenal.1984.247.2.f291
发表时间：
1984-08
期刊：
The American journal of physiology
影响因子：
--
作者：
S. Sansom;Edward J. Weinman;Roger G. O'Neil
通讯作者：
S. Sansom;Edward J. Weinman;Roger G. O'Neil
心房钠尿因子和 cGMP 抑制培养的肾上皮细胞系 LLC-PK1 中阿米洛利敏感的 Na 转运。
DOI：
10.1016/s0006-291x(86)80498-3
发表时间：
1986
影响因子：
3.1
作者：
Cantiello,HF;Ausiello,DA
通讯作者：
Ausiello,DA
DOI：
10.1152/ajprenal.1985.248.4.f500
发表时间：
1985
期刊：
The American journal of physiology
影响因子：
--
作者：
Koeppen,BM
通讯作者：
Koeppen,BM
水、尿素、钠、氯和钾在体外分离的灌注乳头集合管中的转运。
影响因子：
19.6
作者：
Antonino S. Rocha;Lúcia H. Kudo
通讯作者：
Lúcia H. Kudo