Direct measurement of interaction forces between a yeast cell and a microbubble using atomic force microscopy
Direct measurement of interaction forces between a yeast cell and a microbubble using atomic force microscopy
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使用原子力显微镜直接测量酵母细胞和微泡之间的相互作用力
DOI：
10.1016/j.colsurfa.2019.123963
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Nomura Toshiyuki
中科院分区：
文献类型：
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作者：
Yumiyama Shohei;Kato Satoshi;Konishi Yasuhiro;Nomura Toshiyuki
去除表面上粘附的细胞是每个工业过程中的重要问题。尽管由于其积极的特性，越来越多地考虑将气泡用于清洁技术，但使用气泡的清洁效果及其与相互作用力相关的机制仍然知之甚少。原子力显微镜（AFM）具有纳米级的力分辨率，并已越来越多地应用于测量针对气泡或生物表面的相互作用力。然而，很少有研究报道了一个单一的微生物细胞和疏水气泡表面之间的相互作用力的定量测量。在这项研究中，我们试图进行直接测量酵母细胞和微泡（MB）之间的相互作用力，使用原子力显微镜。我们证明了强粘附力归因于酵母细胞和MB之间的毛细管力和疏水力。发现该力大于酵母细胞和不锈钢基底之间的力。这些发现为使用MB的各种应用提供了有用的信息，包括工业过程的清洁技术。
The removal of adhered cells on surfaces is an important issue in every industrial process. Although bubbles are increasingly being considered for use in cleaning technology due to their positive properties, the cleaning effects of using bubbles and their mechanisms related to interaction forces remain poorly understood. Atomic force microscopy (AFM) is capable of nanoscale force resolution and has been increasingly applied to measure the interaction forces targeting bubbles or biological surfaces. However, few studies have reported on the quantitative measurement of interaction forces between a single microbial cell and a hydrophobic bubble surface. In this study, we attempted to conduct direct measurement of the interaction forces between a yeast cell and a microbubble (MB) using AFM. We demonstrated that the strong adhesive force is attributed to the capillary and hydrophobic forces between a yeast cell and a MB. This force was found to be larger than that between a yeast cell and a stainless-steel substrate. These findings provide useful information for various applications using MBs, including cleaning technology for industrial processes.