Expression and Significance of Th17 Cells and Related Factors in Patients with Autoimmune Hepatitis
Expression and Significance of Th17 Cells and Related Factors in Patients with Autoimmune Hepatitis
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DOI：
10.2174/1386207322666190402160455
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发表时间：
2019-01-01
影响因子：
1.8
通讯作者：
An, Jihong
中科院分区：
文献类型：
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作者：
An, Jihong
目的：探讨自身免疫性肝炎(AIH)患者外周血中Th17细胞及相关因素的表达及其临床意义。方法：回顾性选择100例自身免疫性肝炎患者为研究组，并以门诊健康志愿者100例为对照组。结果：观察组血清总胆红素(TBil)、碱性磷酸酶(ALP)、γ-谷氨酰转肽酶(Gamma-GlamylTransspeptidase，GT)、免疫球蛋白G(Ig G)、免疫球蛋白M(Ig M)、丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)水平均高于对照组血清IL-21和肿瘤坏死因子-α水平。研究组外周血中Th17细胞比例较高，Treg细胞比例较低。血清IL-17、TNF-α水平及Th17细胞频率与ALT、AST呈正相关，而Treg细胞频率与ALT、AST水平呈负相关。结论：Th17细胞频率及其相关因子IL-17、TNF-α与肝损害密切相关，可作为监测空气病严重程度的指标。
Objective: This study aims to investigate the expression and clinical significance of Th17 cells and related factors in peripheral blood of patients with Autoimmune Hepatitis (AIH).Methods: A retrospective selection of 100 patients with AIH were included as a study group, and 100 healthy volunteers in the outpatient clinic were selected as the control group. The levels of IL-17, IL-6, IL-21 and TNF-alpha in peripheral blood of all subjects were detected by enzyme-linked immunosorbent assay and the frequency of Th17 cells and Treg cells was detected by flow cytometry.Results: Results showed that the study group had higher levels of serum total bilirubin (TBil), alkaline phosphatase (ALP), gamma -glutamyltranspeptidase (gamma-GT), immunoglobulin G (IgG), immunoglobulin M (IgM), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) than the control group, as well as higher levels of IL-17, IL-6, IL-21 and TNF-alpha in serum. The frequency of Th17 cells in peripheral blood was higher in the study group, while the frequency of Treg cells was lower. Also, serum IL-17, TNF-alpha levels and Th17 cells frequency were positively correlated with ALT and AST, whereas Treg cells frequency were negatively correlated with ALT and AST levels.Conclusion: Our finding demonstrates that Th17 cell frequency and their related factors IL-17 and TNF-alpha, are associated with liver damage, which might be used to monitor AIR disease severity.