Formation of supported lipid bilayers of Escherichia coli extracted lipids and their surface morphologies
Formation of supported lipid bilayers of Escherichia coli extracted lipids and their surface morphologies
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DOI：
10.7567/1347-4065/ab1b5f
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发表时间：
2019-08-01
影响因子：
1.5
通讯作者：
Tero, Ryugo
中科院分区：
文献类型：
--
作者：
Kakimoto, Yasuhiro;Tero, Ryugo
作为细菌模型细胞系统，重组大肠杆菌（E.大肠杆菌）细胞膜。化学提取E.大肠杆菌脂质混合物如极性脂质提取物（PLE）在以前的研究中被广泛使用。这些脂质提取物含有三种主要的磷脂类，磷脂酰乙醇胺（PE）、磷脂酰甘油（PG）和心磷脂（CA）。这些脂质具有不同的电学和物理性质。然而，由各组分组成的脂质双层膜的特性尚未被研究。了解每个脂质提取物组分的行为导致制造细菌模型膜平台，并进一步了解脂质在细菌膜反应中的作用。在这项研究中，我们研究了支持的脂质双层的E。用荧光显微镜和原子力显微镜观察了大肠杆菌在热氧化SiO2/Si和云母基底上提取的PE、PG和CA。(C)2019日本应用物理学会
As a bacterial model cell system, a reconstituted Escherichia coli (E. coli) cell membrane has been studied. The chemically extracted E. coli lipid mixture such as polar lipid extract (PLE) is widely used in the previous studies. These lipid extracts contain three main phospholipid classes, phosphatidylethanolamine (PE), phosphatidylglycerol (PG) and cardiolipin (CA). These lipids have different electrical and physical properties. However, the characters of the lipid bilayer membrane composed of each component have not been investigated. Understanding the behaviors of each lipid extract component leads to fabricating bacterial model membrane platforms, and to further understanding of roles of lipids in bacterial membrane reactions. In this study, we examined supported lipid bilayers of E. coli extracted PE, PG and CA on thermally oxidized SiO2/Si and mica substrates with fluorescence microscopy and atomic force microscopy. (C) 2019 The Japan Society of Applied Physics