Functional assessments in mice and rats after focal stroke
Functional assessments in mice and rats after focal stroke
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DOI：
10.1016/s0028-3908(99)00262-2
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发表时间：
2000-01-01
影响因子：
4.7
通讯作者：
Parsons, AA
中科院分区：
文献类型：
--
作者：
Hunter, AJ;Hatcher, J;Parsons, AA
本文提出了一个全面的评估感觉运动缺陷：在小鼠局灶性脑缺血引起的大脑中动脉闭塞。诱导大脑中动脉闭塞后24小时，小鼠在一系列感觉和运动任务中表现出缺陷，如通过SHIRPA方案评估的。此外，他们的转棒表现和运动活动也有所下降。与正常对照组相比，假手术组动物的一些行为（如自发活动）也受损，尽管这些损伤不像缺血小鼠表现出的那样明显。这是第一次对小鼠永久性局灶性缺血的短期影响进行全面分析。在大鼠的第二系列实验中，检查了经过一段时间的恢复率。在大鼠大脑中动脉闭塞后至缺血后7天检查简单（神经学分级，旋转棒）和复杂（粘性标签测试）任务。缺血大鼠：在粘性标签任务上对侧表现严重缺陷，无恢复证据。一个不太明显的赤字，也观察到在同侧性能的这项任务。缺血后7天，这些缺陷仍然存在。缺血大鼠也表现出转棒性能缺陷，但在缺血后7天恢复。因此，大鼠局灶性脑缺血后不同的感觉运动任务有不同的恢复率。这些任务的进一步表征将增强其效用，评估潜在神经保护和再生疗法的施用的功能恢复的有意义的临床前手段。(C)2000爱思唯尔科技有限公司版权所有。
This paper presents a comprehensive assessment of sensorimotor deficits: in the mouse after focal ischaemia induced by occlusion of the middle cerebral artery. Twenty four hours after induction of middle cerebral artery occlusion, mice showed deficits in a range of sensory and motor tasks as assessed by the SHIRPA protocol. In addition they exhibited a decrease in rotarod performance and locomotor activity. Some behaviours, such as locomotor activity, were also impaired in sham operated animals compared to normal controls, although these impairments were not as marked as those exhibited by the ischaemic mice. This is the first comprehensive analysis of the short term effects of permanent focal ischaemia in mice. In a second series of experiments in the rat, rates of recovery over lime were examined. Simple (neurological grades, rotarod) and complex (sticky label test) tasks were examined in rats after middle cerebral artery occlusion up to 7 days post-ischaemia. Ischaemic rats: had a profound deficit in contralateral performance on the sticky label task with no evidence of recovery. A less marked deficit was also observed in ipsilateral performance of this task. These deficits were still present 7 days after ischaemia. Ischaemic rats also exhibited a deficit on rotarod performance but this had recovered 7 days post-ischaemia. Thus different sensorimotor tasks have different rates of recovery after focal cerebral ischaemia in the rat. Further characterisation of these tasks will enhance their utility meaningful preclinical means of assessing functional recovery of the administration of potential neuroprotective and regenerative therapies. (C) 2000 Elsevier Science Ltd. All rights reserved.