Bax mitochondrial relocation is linked to its phosphorylation and its interaction with Bcl-xL.
Bax mitochondrial relocation is linked to its phosphorylation and its interaction with Bcl-xL.
复制标题
DOI：
10.15698/mic2016.12.547
复制
发表时间：
2016-12-05
期刊：
影响因子：
--
通讯作者：
Manon S
中科院分区：
文献类型：
--
作者：
Garenne D;Renault TT;Manon S
Bax和其他Bcl2家族成员在酿酒酵母中的异源表达，已被证明是研究它们与线粒体相互作用的分子机制的有价值的报告系统。通过对Bax和Bclxl突变体的共表达以及它们在线粒体和线粒体后上清液中的定位和相互作用的分析，我们发现Bax和Bclxl的相互作用能力既依赖于Bax的磷酸化(通过S184D替换来模拟)，也依赖于Bax和Bclxl的定位。这一点和以前的数据为Bax定位和激活的动态平衡模型提供了分子基础，该模型受磷酸化和Bclxl的调节。
The heterologous expression of Bax, and other Bcl-2 family members, in the yeast Saccharomyces cerevisiae, has proved to be a valuable reporter system to investigate the molecular mechanisms underlying their interaction with mitochondria. By combining the co-expression of Bax and Bcl-xL mutants with analyzes of their localization and interaction in mitochondria and post-mitochondrial supernatants, we showed that the ability of Bax and Bcl-xL to interact is dependent both on Bax phosphorylation - mimicked by a substitution S184D - and by Bax and Bcl-xL localization. This, and previous data, provide the molecular basis for a model of dynamic equilibrium for Bax localization and activation, regulated both by phosphorylation and Bcl-xL.