Characterisation of cell adhesion in airway epithelial cell types using electric cell-substrate impedance sensing
Characterisation of cell adhesion in airway epithelial cell types using electric cell-substrate impedance sensing
复制标题
DOI：
10.1183/09031936.00065809
复制
发表时间：
2010-04-01
影响因子：
24.3
通讯作者：
van Oosterhout, A. J. M.
中科院分区：
文献类型：
--
作者：
Heijink, I. H.;Brandenburg, S. M.;van Oosterhout, A. J. M.
为了研究哮喘和慢性阻塞性肺病等呼吸系统疾病中气道上皮细胞结构异常的机制，需要对上皮细胞系和原代上皮进行研究，并采用新型的电细胞基质阻抗传感技术监测细胞粘附/扩散、屏障功能和创伤愈合。将原代支气管上皮与气道上皮细胞系16 HBE 14 o-、BEAS-2B、NCI-H292和A549进行比较。BEAS-2B、A549和原代细胞比16 HBE 14 o-细胞更快地形成融合单层。相比之下，16 HBE 140-细胞形成更强的细胞间接触，其抗性比BEAS-2B、A549和NCI-H292细胞高10倍，比原代细胞高5倍。因此，粘附分子E-occludens-1和E-cadherin的表达在16 HBE 140-细胞中最高。这些分子定位于16 HBE 14 o和原代细胞的细胞间连接处。最后，损伤后屏障功能的恢复受损BEAS-2B相比，16 HBE 14 o-celles.In结论，上皮细胞类型显示显着的表型差异，因此，应被用来解决具体的研究问题。16 HBE 140-细胞似乎最适合于屏障形成的研究，而在原代和BEAS-2B和A549细胞的附着的相似性，使后者更重要的细胞-基质接触的转化研究。
Research on epithelial cell lines and primary epithelium is required to dissect the mechanisms underlying the structural abnormalities in airway epithelium observed for respiratory diseases, including asthma and chronic obstructive pulmonary disease.The novel electric cell-substrate impedance sensing technique was used to monitor cell adhesion/spreading, barrier function and wound healing. Primary bronchial epithelium was compared with airway epithelial cell lines 16HBE14o-, BEAS-2B, NCI-H292 and A549.BEAS-2B, A549 and primary cells form a confluent monolayer more rapidly than do 16HBE14o-cells. In contrast, 16HBE14o- cells form stronger intercellular contacts, with a 10-fold higher resistance than BEAS-2B, A549 and NCI-H292 cells and a five-fold increase over primary cells. Accordingly, expression of the adhesion molecules zona occludens-1 and E-cadherin was highest in 16HBE14o- cells. These molecules were localised in intercellular junctions in both 16HBE14o-and primary cells. Finally, restoration of barrier function upon injury was impaired in BEAS-2B compared to 16HBE14o- cells.In conclusion, epithelial cell types display remarkable phenotypic differences and should, accordingly, be used to address specific research questions. 16HBE14o- cells appear most suitable for studies on barrier formation, whereas resemblance in attachment of primary and BEAS-2B and A549 cells makes the latter more important for translational research on cell-matrix contact.