Reactive free radical generation in vivo in heart and liver of ethanol-fed rats: correlation with radical formation in vitro.
Reactive free radical generation in vivo in heart and liver of ethanol-fed rats: correlation with radical formation in vitro.
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DOI：
10.1073/pnas.84.24.9223
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发表时间：
1987-12
影响因子：
11.1
通讯作者：
L. A. Reinke;E. Lai;C. M. Dubose;P. McCay
中科院分区：
文献类型：
--
作者：
L. A. Reinke;E. Lai;C. M. Dubose;P. McCay
高脂乙醇饮食2周后，大鼠体内肝脏和心脏均产生自由基。这些自由基被认为是碳中心自由基，通过给大鼠注射自旋诱捕剂来检测，并通过电子顺磁共振波谱法进行了表征。肝脏中的自由基被证明局限于内质网。用低脂乙醇喂养的大鼠自由基生成明显减少。对照组动物给予不含乙醇的等热量饮食，肝脏和心脏中没有自由基的迹象。将饲喂高脂乙醇饲料的大鼠制备的肝微粒体放在含有乙醇、NADPH和自旋捕获剂的系统中孵育，观察1-羟乙基自由基的产生。后者用13c取代乙醇进行了验证。喂食高脂肪含乙醇饲料的小鼠的微粒体中细胞色素P-450的水平高于喂食低脂肪含乙醇饲料的小鼠的微粒体。结果表明，乙醇的消耗导致体内大鼠肝脏和心脏产生自由基，这似乎启动了脂质过氧化。
Rats fed a high-fat ethanol-containing diet for 2 weeks were found to generate free radicals in liver and heart in vivo. The radicals are believed to be carbon-centered radicals, were detected by administering spin-trapping agents to the rats, and were characterized by electron paramagnetic resonance spectroscopy. The radicals in the liver were demonstrated to be localized in the endoplasmic reticulum. Rats fed ethanol in a low-fat diet showed significantly less free radical generation. Control animals given isocaloric diets without ethanol showed no evidence of free radicals in liver and heart. When liver microsomes prepared from rats fed the high-fat ethanol diet were incubated in a system containing ethanol, NADPH, and a spin-trapping agent, the generation of 1-hydroxyethyl radicals was observed. The latter was verified by using 13C-substituted ethanol. Microsomes from animals fed the high-fat ethanol-containing diet had higher levels of cytochrome P-450 than microsomes from rats fed the low-fat ethanol-containing diet. The results suggest that the consumption of ethanol results in the production of free radicals in rat liver and heart in vivo that appear to initiate lipid peroxidation.