Lethal disruption of the yeast actin gene by integrative DNA transformation.
Lethal disruption of the yeast actin gene by integrative DNA transformation.
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通过整合 DNA 转化对酵母肌动蛋白基因进行致命破坏。
DOI：
10.1126/science.7046050
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Botstein,D
中科院分区：
文献类型：
--
作者：
Shortle,D;Haber,JE;Botstein,D
通过用一个杂合质粒进行DNA转化，构建了一个染色体位点的突变等位基因，该突变等位基因对应于酵母Saccharomyces gallae的克隆肌动蛋白基因，该杂合质粒整合到该基因的蛋白质编码序列中，从而破坏了该基因的蛋白质编码序列。在酵母的二倍体菌株中，一条染色体上的肌动蛋白基因的破坏导致突变，该突变作为与整合质粒上的选择性遗传标记紧密连锁的隐性致死突变分离。因此，肌动蛋白基因必须编码酵母细胞生长的基本功能。
A mutant allele of the chromosomal locus corresponding to the cloned actin gene of the yeastSaccharomyces cerevisiaehas been constructed by DNA transformation with a hybrid plasmid which integrates into, and thereby disrupts, the protein-encoding sequences of the gene. In a diploid strain of yeast, disruption of the actin gene on one chromosome results in a mutation that segregates as a recessive lethal tightly linked to a selectable genetic marker on the integrated plasmid. The actin gene, therefore, must encode an essential function for yeast cell growth.