Enantioselective Effect of Flutriafol on Growth, Deoxynivalenol Production, and TRI Gene Transcript Levels in Fusarium graminearum
Enantioselective Effect of Flutriafol on Growth, Deoxynivalenol Production, and TRI Gene Transcript Levels in Fusarium graminearum
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Flutriafol 对禾谷镰刀菌生长、脱氧雪腐镰刀菌烯醇生产和 TRI 基因转录水平的对映选择性影响。
DOI：
10.1021/acs.jafc.0c06800
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发表时间：
2021-02-01
影响因子：
6.1
通讯作者：
Liu, Chenglan
中科院分区：
文献类型：
--
作者：
Li, Chaofeng;Fan, Shuai;Liu, Chenglan
近年来，小麦籽粒及其制品中经常检测到脱氧雪腐镰刀菌烯醇(DON)。研究了小麦基质中水分活度(α(W)、0.97和0.99)和温度(20、25和30℃)对氟曲胺醇对禾谷镰刀菌生长和DON生物合成的对映选择性影响。在上述条件下，R-(-)-氟曲酚的生物活性高于S-(+)-氟曲酚和RAc-氟曲酚。氟曲胺醇对映体减少或刺激DON的生物合成依赖于α(W)。在0.97 a(W)和0.99 a(W)下孵育14天和7天后，DON水平可忽略不计。实时定量聚合酶链式反应(qRT-PCR)分析表明，禾谷镰刀菌在0.97a(W)下的生物合成(Tri)基因表达水平显著高于0.99a(W)下的表达水平。此外，R-(-)-氟曲酚比S-(+)-氟曲酚能诱导更多的tri基因表达。综上所述，本研究表明Aw和温度在调节禾谷镰刀菌对氟曲霉醇对映体的DON生物合成中起重要作用。
In recent years, deoxynivalenol (DON) has frequently been detected in wheat grains and their products. The enantioselective impact of flutriafol on the growth and DON biosynthesis of Fusarium graminearum was investigated in relation to water activity (alpha(w), 0.97 and 0.99) and temperature (20, 25, and 30 degrees C) on the wheat-based medium. R-(-)-flutriafol exhibited higher bioactivity than S-(+)-flutriafol and Rac-flutriafol under the above conditions. Flutriafol enantiomers reduced or stimulated DON biosynthesis depending on alpha(w). DON levels were negligible after 14 or 7 days of incubation times under 0.97 and 0.99 a(w), respectively. Quantitative real-time polymerase chain reaction (qRT-PCR) analyses showed that the expression levels of trichothecene biosynthetic (TRI) genes of F. graminearum under 0.97 a(w) were significantly higher than those under 0.99 a(w). In addition, R-(-)-flutriafol can induce more TRI gene expression than S-(+)-flutriafol. Taken together, this study indicated that aw and temperature play important roles in regulating DON biosynthesis in F. graminearum with flutriafol enantiomers.