AGE-DEPENDENT CHANGES IN THE SUBCELLULAR-DISTRIBUTION OF RAT-BRAIN MU-OPIOID RECEPTORS AND GTP BINDING REGULATORY PROTEINS
AGE-DEPENDENT CHANGES IN THE SUBCELLULAR-DISTRIBUTION OF RAT-BRAIN MU-OPIOID RECEPTORS AND GTP BINDING REGULATORY PROTEINS
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DOI：
10.1111/j.1471-4159.1991.tb06340.x
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发表时间：
1991-11-01
影响因子：
4.7
通讯作者：
COSCIA, CJ
中科院分区：
文献类型：
--
作者：
BEM, WT;YEUNG, SJ;COSCIA, CJ
比较了1日龄和成年大鼠前脑μ阿片受体和鸟嘌呤核苷酸结合调节蛋白（G蛋白）的相对亚细胞分布。 通过蔗糖密度梯度离心将轻膜（LM）与重膜（HM）分离。 标记酶分析表明，LMs包含了大部分的内质网和高尔基复合体，而HMs丰富的质膜。 使用[H-3][D-Ala 2，Me-Phe 4，Gly-ol 5]脑啡肽评估μ-阿片样物质位点的结合分布和性质。 P1 LMs具有43%的总μ-阿片样物质结合检测相比，16%的成人。 虽然NaCl抑制亩结合在LM在更大程度上比HM，年龄依赖性差异没有观察到。 P1 LM mu结合对5 '-鸟苷酰亚胺二磷酸的敏感性比它们的成年对应物更高。 此外，P1 LMs含有更多的G（o-α）蛋白比P1 HMs或成人LMs，证明了免疫印迹抗血清抗G（o-α）后，一维或二维凝胶电泳。 这些结果表明，P1 LMs含有更大比例的新合成的细胞内亩网站比成人LMs。
The relative subcellular distributions of mu-opioid receptors and guanine nucleotide binding regulatory proteins (G proteins) in 1-day-old (P1) and adult rat forebrain were compared. Light membranes (LMs) were resolved from heavy membranes (HM) by sucrose density gradient centrifugation. Marker enzyme analyses indicated that LMs contained most of the endoplasmic reticulum and Golgi complexes, whereas HMs were enriched in plasma membranes. Binding distribution and properties of mu-opioid sites were assessed using [H-3][D-Ala2,Me-Phe4,Gly-ol5]enkephalin. P1 LMs possessed 43% of the total mu-opioid binding detected compared to 16% in the adult. Although NaCl inhibited mu binding in LMs to a greater extent than in HMs, age-dependent differences were not observed. P1 LM mu binding possessed greater sensitivity to 5'-guanylylimdodiphosphate than their adult counterpart. Moreover, P1 LMs contained more G(o-alpha) protein than P1 HMs or adult LMs, as demonstrated by immunoblotting with antisera against G(o-alpha) after one- or two-dimensional gel electrophoresis. These results suggest that P1 LMs contain a greater proportion of newly synthesized intracellular mu sites than adult LMs.