Isolation and characterization of the gene coding for the major sigma factor of Rickettsia prowazekii DNA-dependent RNA polymerase.
Isolation and characterization of the gene coding for the major sigma factor of Rickettsia prowazekii DNA-dependent RNA polymerase.
复制标题
普瓦泽基立克次体 DNA 依赖性 RNA 聚合酶主要 sigma 因子编码基因的分离和表征。
DOI：
10.1016/0378-1119(92)90175-o
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发表时间：
1992
期刊：
影响因子：
3.5
通讯作者：
Wood,DO
中科院分区：
文献类型：
--
作者：
Marks,GL;Winkler,HH;Wood,DO
利用寡脱氧核苷酸作为探针，在主要σ因子的保守区，分离了普氏立克次体主要σ因子的编码基因。核苷酸序列分析表明，该基因的开放阅读框为1905 bp，编码635个氨基酸，分子量为73 kDa（σ73）。prowazekiiσ 73与多种真细菌的主要σ因子具有广泛的同源性。大肠杆菌和R. prowazekii的氨基酸同源性为44.9%; prowazekiiσ 73在E.十二烷基硫酸钠聚丙烯酰胺凝胶电泳分析表明，大肠杆菌亚胺细胞迁移为85-kDa蛋白。这种异常迁移是真细菌主要σ因子的特征，并与纯化的立克次体σ蛋白的迁移一致。尽管与E相似。coliσ 70编码的rpoD、R. prowazekiiσ 73与E. colirpoD温度敏感突变体。
The gene coding for the major σ factor ofRickettsia prowazekii, an obligate intracellular parasitic bacterium, has been isolated utilizing an oligodeoxyribonucleotide as a probe to a conserved region of major σ factors. Nucleotide sequence analysis revealed an open reading frame of 1905 bp that could encode a protein of 635 amino acids (aa) with a calculated molecular size of 73 kDa (σ73).R. prowazekiiσ73displayed extensive homology with major σ factors from a variety of eubacteria. Comparison of the major σ factors fromEscherichia coliandR. prowazekiirevealed 44.9% aa identity.R. prowazekiiσ73produced inE. coliminicells migrated as a 85-kDa protein when analyzed by sodium dodecyl sulfatepolyacrylamide-gel electrophoresis. This anomalous migration is characteristic of eubacterial major σ factors and agrees with the migration noted for the purified rickettsial σ protein. Despite a similarity to theE. coliσ70encoded byrpoD, R. prowazekiiσ73did not complementE. coli rpoDtemperature-sensitive mutants.