Kalemotropic effect of epinephrine: analysis with adrenergic agonists and antagonists.
Kalemotropic effect of epinephrine: analysis with adrenergic agonists and antagonists.
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肾上腺素的促钾作用：肾上腺素能激动剂和拮抗剂的分析。
DOI：
10.1152/ajplegacy.1971.220.6.1964
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发表时间：
1971
期刊：
影响因子：
--
通讯作者：
R. Vick
中科院分区：
文献类型：
--
作者：
E. P. Todd;R. Vick
方法体重12 ~ 31 kg，性别和品种未选择的成年猪，用硫酸吗啡10 mg/kg皮下注射麻醉，然后用戊巴比妥钠15 mg/kg静脉注射30 min。插入气管插管，使用活塞式气泵控制呼吸。在颈部分离两条迷走神经并切断，以减少动脉压变化时固有血管活性的反射变化。这一程序已被证明不影响血浆中的变化[K]引起的eipinealdehyde（2 - 1）。记录股动脉压和Ⅱ导联心电图，用95 ℃乙醇溶液（50 mg/ml）静脉滴注肾上腺素能α受体拮抗剂盐酸酚苄明（5 mg/kg），用30 mg/ml水溶液静脉滴注肾上腺素能β受体拮抗剂盐酸普萘洛尔（3 mg/kg）。以该速率（0.01 ml/kg/min）单独输注每种溶媒未产生可检测到的影响（35）。之间的时间间隔开始的输液和管理的cpinclaminc是：苯苄明，60分钟，普萘洛尔，30分钟。当两种拮抗剂给予tQ相同的动物，苯苄明前普萘洛尔。每种拮抗剂和肾上腺素输注之间的间隔与上述相同。
METHODSDogs weighing between 12 and 31 kg and not selected for sex or breed were anesthetized with a combination of morphine sulfate, 10 rng/kg, SC, followed in 30 min by pentobarbital sodium, 15 mg/kg, iv. A tracheal cannula was inserted and a piston-type air pump was used to control respiration. Both vagus nerves were isolated in the neck and cut to reduce reflex changes of intrinsic vasomotor activity when arterial pressure changed. This procedure has been shown not to affect the changes in plasma [K] caused by eipinephrine (2 1). Femoral arterial pressure and lead II ECG were recorded.The adrenergic a-receptor antagonist phenoxybenzamine HCl was administered (5 mg/kg) in 95 c/c ethanol solution (50 mg/ml), and the adrenergic, &receptor antagonist propranolol HCl was administered (3 rng/kg) in aqueous solution (30 mg,/ml) by intravenous infusion during a lo-min period. The infusion of each of the vehicles alone at this rate (0.01 ml/kg per min) produced no detectable effects (35). The interval between the beginning of the infusion and the administration of cpincphrine was: phenoxybenzamine, 60 min, propranolol, 30 min. When both antagonists were given tQ the same animal, phenoxybenzaminc was administered before propranolol. The interval between the infusion of each antagonist and epinephrine was the same as given above.