Lysosomal changes during thyroxine-induced left ventricular hypertrophy in rabbits.
Lysosomal changes during thyroxine-induced left ventricular hypertrophy in rabbits.
复制标题
甲状腺素诱导的兔左心室肥大过程中溶酶体的变化。
DOI：
10.1152/ajpcell.1986.251.5.c737
复制
发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Decker,RS
中科院分区：
文献类型：
--
作者：
Parmacek,MS;Decker,ML;Lesch,M;Samarel,AM;Decker,RS
通过使用形态学、免疫细胞化学和生化相结合的方法，本研究证明了伴随甲状腺素诱导的心脏肥大的溶酶体空泡装置的变化。在施用甲状腺素的第一周期间，免疫细胞化学研究显示许多肌细胞内的组织蛋白酶 D 下降，但间质细胞内的组织蛋白酶 D 增加。这些事件发生时组织蛋白酶 D 活性仅略有上升。在第 2 周期间，组织蛋白酶 D 在大多数肌细胞中重新出现，并在间质群体中继续增加，并且与溶酶体酶活性的普遍升高相关。这些数据证明了采用免疫细胞化学和生物化学方法来评估溶酶体液泡装置状态的重要性，并表明溶酶体液泡装置可能参与了伴随心脏快速生长的明显重塑，这可能代表了该心脏肥大模型中记录的蛋白水解速率增强的一个组成部分。
By use of a combined morphologic, immunocytochemical, and biochemical approach, this study demonstrates the changes in the lysosomal vacuolar apparatus that accompany thyroxine-induced cardiac hypertrophy. During the 1st wk of thyroxine administration, immunocytochemical studies revealed a decline in cathepsin D within many myocytes, but an increase within interstitial cells. These events transpired with only a modest rise in cathepsin D activity. During the 2nd wk, cathepsin D reappeared within most myocytes and continued to increase within the interstitial population and was associated with a general rise in lysosomal enzyme activity. These data demonstrate the importance of employing both immunocytochemical and biochemical approaches to evaluate the status of the lysosomal vacuolar apparatus, and suggests that the lysosomal vacuolar apparatus may be involved in the apparent remodeling that attends rapid cardiac growth, which may represent one component of the enhanced rates of proteolysis documented in this model of cardiac hypertrophy.