Stress-Induced Hyperthermia in Singly Housed Mice
Stress-Induced Hyperthermia in Singly Housed Mice
复制标题
压力引起的单养小鼠体温过高
DOI：
10.1016/s0031-9384(97)00157-1
复制
发表时间：
1997
影响因子：
2.9
通讯作者：
B. Olivier
中科院分区：
文献类型：
--
作者：
J. A. Heyden;T. Zethof;B. Olivier
Van Der Heyden、J. A. M.、T. J. J. Zethof 和 B. Olivier。压力引起的单养小鼠体温过高。 Physiol Behav 62(3) 463–470, 1997。-群养小鼠的应激诱导高热 (SIH) 范例可以筛选假定的抗焦虑药物。群养 SIH 适应于单独饲养的动物，以大幅减少使用的动物数量。测量了各种压力源对直肠温度的影响，以找到简单可靠的测试程序。对动物的重复而非单次干扰会在 10 分钟内导致强烈的体温过高 (ΔT)。固定 1 分钟或直肠温度测量本身后获得类似的热反应。 120dB 的声刺激和重复的 1mA 足部电击都不会导致温度变化，但 2mA 电足部电击会导致体温过高。最终选择的测试范例包括以 10 分钟的间隔重复测量温度，从而在短时间内提供每只动物的基础温度和 ΔT 信息。以 10 分钟为间隔重复进行温度测量，结果显示大约 30 分钟后出现最高体温，但在第一次测量后 10 分钟就已经出现了高达 70% 的高体温。连续每天或每周重复使用动物导致基础温度和 10 分钟后温度逐渐升高。在较短的测试间间隔（一天）下，ΔT 也下降，而每周间隔并不影响 ΔT 的幅度。预先注射动物会导致适度的体温过高，60 分钟后恢复到基线。抗焦虑药地西泮和 5-HT1A 受体激动剂氟辛克生剂量依赖性地抑制 SIH。抗抑郁药阿米替林降低了体温水平，但不影响 ΔT。单养小鼠的 SIH 模型似乎是一种快速且可重复的抗焦虑活性筛选试验。与集体饲养的版本相比，单独饲养的 SIH 大大减少了使用的动物数量。
Van Der Heyden, J. A. M., T. J. J. Zethof and B. Olivier. Stress-induced hyperthermia in singly housed mice. Physiol Behav 62(3) 463–470, 1997.—The stress-induced hyperthermia (SIH) paradigm in group-housed mice allows screening of putative anxiolytic drugs. The group-housed SIH was adapted to singly housed animals in order to drastically reduce the number of animals used. The effect of various stressors on rectal temperature was measured in order to find a simple and reliable test procedure. Repeated, but not single disturbance of animals resulted in a strong hyperthermia (ΔT) within 10 min. Similar hyperthermic responses were obtained after immobilization for 1 min or rectal temperature measurement itself. Neither a 120dB acoustic stimulus, nor repeated 1mA footshocks led to a temperature change, but 2 mA electric footshocks led to hyperthermia. The final test paradigm chosen involved repeated temperature measurement at a 10 min interval, thus providing both information on basal temperature and ΔT in each animal within a short time frame. Repeated temperature measurements at 10 min intervals revealed a maximum hyperthermia after approximately 30 min, but up to 70% of the hyperthermia is already present 10 min after the first measurement. Repeated use of animals at successive daily or weekly intervals resulted in a gradual increase of both the basal temperature and the temperature 10 min later. At short inter-test intervals (one day) ΔT also decreased, whereas weekly intervals did not affect the amplitude of ΔT. Prior injection of the animals resulted in modest hyperthermia, that returned to baseline after 60 min. The anxiolytics diazepam and 5-HT1Areceptor agonist flesinoxan dose-dependently suppressed SIH. The antidepressant amitriptyline lowered temperature levels but did not affect ΔT. The SIH model in singly housed mice appears a fast and reproducible screening test for anxiolytic activity. Compared to the group-housed version, the singly-housed SIH enabled a drastic reduction in the number of animals used.