Minimally invasive microendoscopy system for in vivo functional imaging of deep nuclei in the mouse brain
Minimally invasive microendoscopy system for in vivo functional imaging of deep nuclei in the mouse brain
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DOI：
10.1364/boe.6.004546
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发表时间：
2015-11-01
影响因子：
3.4
通讯作者：
Aponte, Yeka
中科院分区：
文献类型：
--
作者：
Bocarsly, Miriam E.;Jiang, Wan-chen;Aponte, Yeka
光学显微镜的一个主要目标是能够对哺乳动物大脑中任何地方的神经元进行成像。在这里，我们描述了一种微创的显微内窥镜系统，用于研究深层神经元的形态和功能。利用超薄壁导管，对小鼠脑内深埋核团，如纹状体(2.5 mm深)、黑质(4.4 mm深)和下丘脑外侧核(5.0 mm深)进行了活体双光子荧光成像。我们首次报道了头部固定清醒小鼠下丘脑外侧区和黑质的亚细胞分辨率神经元活动的观察。(C)2015年美国光学学会
The ability to image neurons anywhere in the mammalian brain is a major goal of optical microscopy. Here we describe a minimally invasive microendoscopy system for studying the morphology and function of neurons at depth. Utilizing a guide cannula with an ultrathin wall, we demonstrated in vivo two-photon fluorescence imaging of deeply buried nuclei such as the striatum (2.5 mm depth), substantia nigra (4.4 mm depth) and lateral hypothalamus (5.0 mm depth) in mouse brain. We reported, for the first time, the observation of neuronal activity with subcellular resolution in the lateral hypothalamus and substantia nigra of head-fixed awake mice. (C) 2015 Optical Society of America