Inhibition of spontaneous and opiate-modified nociception by an endogenous neuropeptide with Phe-Met-Arg-Phe-NH2-like immunoreactivity.
Inhibition of spontaneous and opiate-modified nociception by an endogenous neuropeptide with Phe-Met-Arg-Phe-NH2-like immunoreactivity.
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DOI：
10.1073/pnas.81.15.5002
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发表时间：
1984-08
影响因子：
11.1
通讯作者：
J. Tang;H. Y. Yang;E. Costa
中科院分区：
文献类型：
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作者：
J. Tang;H. Y. Yang;E. Costa
大鼠脑室内注射吗啡或鞘内注射[Met5]脑啡肽- arg6 - phe7 （YGGFMRF）经10微升含1微克卡托普利/1微克贝司他汀/2.5微克ph - met - arg - ph - nh2 （FMRF-NH2）的人工脑脊液预处理后，其抗痛觉作用减弱。从牛脑中纯化的高分子量形式的FMRF-NH2减弱了YGGFMRF的抗伤性作用。从FMRF-NH2特异性抗血清中制备的IgG可引起纳洛酮可逆的中度抗痛作用；相比之下，从对照血清中制备的IgG未能改变甩尾潜伏期。在每2小时注射一次吗啡和每4小时注射一次抗fmrf - nh2 IgG的大鼠中，连续注射8次后抗痛觉作用仍然明显；在只接受吗啡的大鼠中，连续注射六次后，抗痛觉作用消失了。在大鼠脊髓蛛网膜下腔灌注液中加入吗啡（1 μ m），在灌注液中释放fmrf - nh2样肽。
In rats the antinociceptive actions of morphine (injected intraventricularly) or of [Met5]enkephalin-Arg6-Phe7 (YGGFMRF) (injected intrathecally) were attenuated by a pretreatment with 10 microliter of artificial cerebral spinal fluid containing 1 microM captopril/1 microM bestatin/2.5 microM Phe-Met-Arg-Phe-NH2 (FMRF-NH2) given 5 min earlier by the same route. A high molecular weight form of FMRF-NH2 purified from bovine brain attenuated the antinociceptive action of YGGFMRF. IgG, prepared from a specific FMRF-NH2 antiserum, elicited a moderate antinociception reversible by naloxone; in contrast, IgG prepared from control serum failed to change tail-flick latencies. In rats receiving morphine every 2 hr and anti-FMRF-NH2 IgG every 4 hr, the antinociceptive action was still evident after eight successive injections; in rats receiving only morphine, the antinociceptive action had disappeared after six successive injections. Morphine (1 microM) added to the perfusion fluid of the subarachnoidal spaces of rat spinal cord releases FMRF-NH2-like peptides in the perfusate.