Chromosome translocation induced by the insertion of the URA blaster into the major repeat sequence (MRS) in Candida albicans.
Chromosome translocation induced by the insertion of the URA blaster into the major repeat sequence (MRS) in Candida albicans.
复制标题
将 URA 冲击波插入白色念珠菌的主要重复序列 (MRS) 中诱导染色体易位。
DOI：
10.1002/yea.1116
复制
发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Suzuki,Takahito
中科院分区：
文献类型：
--
作者：
Iwaguchi,Shin-Ichi;Suzuki,Mina;Sakai,Naomi;Nakagawa,Yoshiyuki;Magee,PaulT;Suzuki,Takahito
Electrophoretic karyotype studies have shown that clinical isolates ofCandida albicanshave extensive chromosome length polymorphisms. Chromosome translocation is one of the causes of karyotypic variation. Chromosome translocation events have been shown to occur very frequently at or near the major repeat sequence (MRS) on chromosomes. The MRS consists of the repeated sequences RB2, RPS and HOK, and the repeated sequences are considered to be the template for recombination. To investigate which element of the MRS is important for chromosome translocation, we constructed three cassettes, each containing aURAblaster and sequences homologous to one of the repeats, for insertion into the MRS region on the chromosomes. Theura3strain STN22u2, which shows a stable, standard karyotype, was transformed with each construct. Insertion events with each cassette occurred at almost all chromosomes. Insertion into the RB2 repeat, but not into the RPS repeat, was accompanied by chromosome translocation in some transformants: chromosome translocations between chromosomes R and 7 and chromosomes 1 and 7 were found, as well as deletions of 7A and 7C from chromosome 7. We conclude that the insertion at the RB2 region may initiate chromosome translocation inC. albicans. Copyright © 2004 John Wiley & Sons, Ltd.
