M13 Virus-Based Framework for High Fluorescence Enhancement
M13 Virus-Based Framework for High Fluorescence Enhancement
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DOI：
10.1002/smll.201901233
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发表时间：
2019-07-01
期刊：
影响因子：
13.3
通讯作者：
Belcher, Angela M.
中科院分区：
文献类型：
--
作者：
Huang, Shengnan;Qi, Jifa;Belcher, Angela M.
荧光成像是研究生物相关大分子的有力工具，但其适用性往往受到荧光探针的限制，荧光探针必须具有高的位点特异性和发射效率。在这方面，M13病毒是一种多用途的生物支架，以前已被用于在其病毒衣壳上以分子精度组装荧光团，并靶向各种细胞。虽然m13 -荧光团系统具有高选择性，但由于低吸收截面和中等量子产率，这些配合物通常具有较差的分子检测极限。为了克服这些挑战，开发了M13病毒、菁3染料和银纳米粒子的共同组装，以创建能够以高发射率的分子精度结合的荧光标签。通过不同的纳米颗粒大小和颗粒-荧光团分离，提高了高达24倍的菁3发射。此外，发现荧光增强随病毒衣壳上染料表面密度的增加而增加。最后，将该高荧光探针应用于大肠杆菌的体外染色。这些结果证明了实现调谐荧光增强的廉价框架。在这项工作中开发的方法可能适用于广泛的M13/细胞组合的荧光检测。
Fluorescence imaging is a powerful tool for studying biologically relevant macromolecules, but its applicability is often limited by the fluorescent probe, which must demonstrate both high site-specificity and emission efficiency. In this regard, M13 virus, a versatile biological scaffold, has previously been used to both assemble fluorophores on its viral capsid with molecular precision and to also target a variety of cells. Although M13-fluorophore systems are highly selective, these complexes typically suffer from poor molecular detection limits due to low absorption cross-sections and moderate quantum yields. To overcome these challenges, a coassembly of the M13 virus, cyanine 3 dye, and silver nanoparticles is developed to create a fluorescent tag capable of binding with molecular precision with high emissivity. Enhanced emission of cyanine 3 of up to 24-fold is achieved by varying nanoparticle size and particle-fluorophore separation. In addition, it is found that the fluorescence enhancement increases with increasing dye surface density on the viral capsid. Finally, this highly fluorescent probe is applied for in vitro staining of E. coli. These results demonstrate an inexpensive framework for achieving tuned fluorescence enhancements. The methodology developed in this work is potentially amendable to fluorescent detection of a wide range of M13/cell combinations.