Wenshen Yangxue decoction (温肾养血方) promotes follicular development in aged female mice via stimulation of the silent information regulator 3/forkhead transcription factor O1 3a pathway
Wenshen Yangxue decoction (温肾养血方) promotes follicular development in aged female mice via stimulation of the silent information regulator 3/forkhead transcription factor O1 3a pathway
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DOI：
10.19852/j.cnki.jtcm.20220617.004
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发表时间：
2022
影响因子：
2.6
通讯作者：
Xin Mingwei
中科院分区：
文献类型：
--
作者：
Zhang Liang;Han Qian;Yi Yanxiao;Ji Shaoyang;Xin Mingwei
OBJECTIVE:To primarily explore the effect and mechanism of Wenshen Yangxue decoction (温肾养血方) in promoting follicular development in elderly female mice.METHODS:Fifty Institute of Cancer Research mice were randomly divided into blank,controlled ovarian hyperstimulation (COH),low-dose Wenshen Yangxue decoction,medium-dose Wenshen Yangxue decoction,and high-dose Wenshen Yangxue decoction groups,with 10 mice in each group.The number of ovulations,number of fertilizations,mitochondrial adenosine triphosphate (ATP) level,and mitochondrial DNA (mtDNA) of oocytes in each group were compared.Reverse transcriptionpolymerase chain reaction and Western blotting were used to detect the mRNA and protein expression levels of silent information regulator 3 (Sirt3) and forkhead transcription factor O1 3a (FOXO3a).RESULTS:Wenshen Yangxue decoction significantly increased the number of ovulations in mice (P <0.05) and promoted the formation of fertilized eggs.The ATP level and mtDNA copy number of mice oocytes in the highdose groups were significantly higher than those in the COH group (P <0.05).Wenshen Yangxue decoction significantly increased the mRNA and protein levels of Sirt3 and FOXO3a in mouse oocytes.CONCLUSION:Wenshen Yangxue decoction promoted the development of follicles in elderly female mice,increased the number of ovulations and improved fertility.Its mechanism may be related to increased mitochondrial energy metabolism and regulation of the Sirt3/FOXO3a pathway.