A pressure-driven capillary electrophoretic system with injection valve sampling.
A pressure-driven capillary electrophoretic system with injection valve sampling.
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DOI：
10.1039/c4an01315h
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发表时间：
2015-01
期刊：
影响因子：
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通讯作者：
Chao Han;Jiannan Sun;Jinhua Liu;Heyong Cheng;Yuan-chao Wang
中科院分区：
文献类型：
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作者：
Chao Han;Jiannan Sun;Jinhua Liu;Heyong Cheng;Yuan-chao Wang
为了提高毛细管电泳（CE）的重复性和效率，简化操作流程，开发了带进样阀的加压毛细管电泳系统（p-CE）。它由一台高压泵、一个六通进样阀、一个PEEK四通、一个分离和背压毛细管、一个紫外可见检测器和一个高压电源组成。分离毛细管中的压力驱动流量范围为 4.5 nL min(-1) 至 0.81 μL min(-1)，是通过分流来自高压泵的流量 (0.005-0.4 mL min(-1)）而产生的。纳体积样品注射（<10 nL）是通过具有分流功能的微体积旋转注射器（0.5-5 μL）进行的。在p-CE系统中，新的商用毛细管可以直接使用，无需任何清洗，并且也消除了运行之间的毛细管冲洗过程。在这种情况下，分析物在加压流、电场力和微小电渗流的作用下被驱向分离毛细管的出口，并由于电泳迁移率而被分离。 p-CE系统允许加压流速和电场独立变化，并在高电场（500-1000 V cm(-1)）和流速梯度模式下实现良好重复性（低于3%）的电泳分离。通过对加碘食盐中的碘酸盐定量，证明了 p-CE 系统在实际分析中的可行性。 0.3 min内实现了碘化物和碘酸盐的分离，证明了其较高的分析速度。
To improve repeatability and efficiency and to simplify the operation procedure of capillary electrophoresis (CE), a pressurized CE system (p-CE) with injection valve sampling was developed. It consisted of one high-pressure pump, a six-port injection valve, a PEEK cross, a separation and back pressure capillary, an ultraviolet-visible detector and a high voltage power supply. The pressure-driven flow ranging from 4.5 nL min(-1) to 0.81 μL min(-1) in the separation capillary was produced by splitting to the flow from the high-pressure pumps (0.005-0.4 mL min(-1)). Nano-volume sample injection (<10 nL) was conducted by a micro-volume rotary injector (0.5-5 μL) with flow splitting. In the p-CE system, the new commercial capillary could be directly used without any wash, and the capillary-flush process between runs was also eliminated. In this case, the analytes were driven toward the outlet of the separation capillary by the pressurized flow, the electric field force and minute electroosmotic flow, and they were separated owing to the electrophoretic mobility. The p-CE system allows for the independent variation of the pressurized flow rate and electrical field and electrophoretic separation of good repeatability (below 3%) under high electrical fields (500-1000 V cm(-1)) and flow rate gradient modes. The feasibility of the p-CE system in real analysis was demonstrated by iodate quantification in iodized table salts. The separation of iodide and iodate was realized within 0.3 min, proving its high analytical speed.