Prognostic potential of PRPF3 in hepatocellular carcinoma
Prognostic potential of PRPF3 in hepatocellular carcinoma
复制标题
PRPF3 在肝细胞癌中的预后潜力。
DOI：
10.18632/aging.102665
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发表时间：
2020-01-15
期刊：
影响因子：
5.2
通讯作者：
Yang, Jin
中科院分区：
文献类型：
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作者：
Liu, Yinlan;Yang, Yuhan;Yang, Jin
前mRNA加工因子3（PRPF 3）是外显子连接复合物的核心组分中的RNA结合蛋白。异常PRPF 3表达可能与癌发生相关。然而，PRPF 3在肝细胞癌（HCC）中的生物学作用仍有待确定。我们通过多个基因表达数据库分析了PRPF 3的表达，并使用cBioPortal鉴定了其遗传改变和功能网络。使用LinkedOmics鉴定与PRPF 3及其调节子共表达的基因。通过肿瘤免疫评估资源（TIMER）研究PRPF 3与癌症免疫浸润的相关性。在多个HCC队列中，发现PRPF 3在肿瘤组织中随着扩增而上调。PRPF 3高表达与较差的总生存期（OS）和无病生存期（DFS）相关。功能网络分析表明，PRPF 3通过多种癌症相关激酶和E2F家族调节剪接体、DNA复制和细胞周期信号传导。值得注意的是，PRPF 3表达与CD 4 + T和CD 8 + T细胞、巨噬细胞、中性粒细胞和树突状细胞的浸润水平正相关。PRPF 3表达与HCC中不同的免疫标志物集有很强的相关性。这些结果提示PRPF 3与肝癌的预后和免疫浸润相关，为进一步研究PRPF 3在肝癌中的免疫调节作用奠定了基础。
pre-mRNA processing factor 3 (PRPF3) is an RNA binding protein in a core component of the exon junction complex. Abnormal PRPF3 expression is potentially associated with carcinogenesis. However, the biological role of PRPF3 in hepatocellular carcinoma (HCC) remains to be determined. We analyzed PRPF3 expression via multiple gene expression databases and identified its genetic alterations and functional networks using cBioPortal. Co-expressed genes with PRPF3 and its regulators were identified using LinkedOmics. The correlations between PRPF3 and cancer immune infiltrates were investigated via Tumor Immune Estimation Resource (TIMER). PRPF3 was found up-regulated with amplification in tumor tissues in multiple HCC cohorts. High PRPF3 expression was associated with poorer overall survival (OS) and disease-free survival (DFS). Functional network analysis suggested that PRPF3 regulates spliceosome, DNA replication, and cell cycle signaling via pathways involving several cancer-related kinases and E2F family. Notably, PRPF3 expression was positively correlated with infiltrating levels of CD4+ T and CD8+ T cells, macrophages, neutrophils, and dendritic cells. PRPF3 expression showed strong correlations with diverse immune marker sets in HCC. These findings suggest that PRPF3 is correlated with prognosis and immune infiltrating in HCC, laying a foundation for further study of the immune regulatory role of PRPF3 in HCC.