Disposable paracetamol sensor based on electroactive molecularly imprinted polymer nanoparticles for plasma monitoring
Disposable paracetamol sensor based on electroactive molecularly imprinted polymer nanoparticles for plasma monitoring
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DOI：
10.1016/j.snb.2020.129128
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发表时间：
2021-01-17
影响因子：
8.4
通讯作者：
Piletsky, Sergey A.
中科院分区：
文献类型：
--
作者：
Alanazi, Kaseb;Cruz, Alvaro Garcia;Piletsky, Sergey A.
利用电活性分子印迹聚合物纳米粒子（nanoMIPs）设计了一种高灵敏度的扑热息痛一次性电化学传感器。采用固相合成法制备了纳米分子印迹聚合物。聚合物组成包括作为特定功能单体的衣康酸和作为氧化还原标记的二茂铁，其赋予纳米颗粒电活性。通过傅里叶变换红外光谱（FTIR）、动态光散射（DLS）和扫描电子显微镜（SEM）对纳米分子印迹聚合物进行了表征。通过将纳米分子印迹聚合物共价连接在丝网印刷碳电极上制备了传感器，并利用差分脉冲伏安法（DPV）对扑热息痛进行了电化学测定。在加标人血浆中成功评价了传感器，回收率为94- 108%，灵敏度为6.18 +/- 0.22 mu A mM（-1）。传感器的检测限和定量限分别为50 μ M和167 μ M，在0.1和1 mM之间的线性浓度范围内。证明了高选择性，在咖啡因，普鲁卡因胺或4-氨基苯甲酸乙酯的存在下没有发现干扰。传感器表现出高再现性（RSD，4.8%），快速响应时间（类似于8秒）和可接受的保质期（90天），确认其适用于护理点诊断应用。
A highly sensitive disposable electrochemical sensor for paracetamol was devised using electroactive molecularly imprinted polymers nanoparticles (nanoMIPs). NanoMIPs were prepared by solid-phase synthesis. Polymer composition included itaconic acid as a specific functional monomer and ferrocene as redox label, which confers electroactivity to the nanoparticles. NanoMIPs were characterised by Fourier transform infrared spectroscopy (FTIR), dynamic light scattering (DLS) and scanning electron microscopy (SEM). Sensors were fabricated by covalent attachment of nanoMIPs on screen-printed carbon electrodes and then employed for electrochemical determination of paracetamol using differential pulse voltammetry (DPV). The sensor was successfully evaluated in spiked human plasma with recoveries at 94-108 % and presenting a sensitivity of 6.18 +/- 0.22 mu A mM(-1). The limit of detection and limit of quantification for the sensor were found to be 50 mu M and 167 mu M, respectively, in a linear concentration range between 0.1 and 1 mM. High selectivity was demonstrated, with no interference found in the presence of caffeine, procainamide or ethyl 4-aminobenzoate. The sensors exhibited high reproducibility (RSD, 4.8 %), fast response time (similar to 8 s) and acceptable shelf life (90 days), confirming its suitability for point of care diagnostic applications.