Pork peptone stimulates cholecystokinin secretion from enteroendocrine cells and suppresses appetite in rats
Pork peptone stimulates cholecystokinin secretion from enteroendocrine cells and suppresses appetite in rats
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DOI：
10.1271/bbb.60046
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发表时间：
2006-08-01
影响因子：
1.6
通讯作者：
Hara, Hiroshi
中科院分区：
文献类型：
--
作者：
Sufian, M. K. N. B.;Hira, Tohru;Hara, Hiroshi
我们发现，大豆β-伴大豆球蛋白胨（BconP）抑制食物的摄入，通过胆囊收缩素（CCK）的释放从肠内分泌细胞与结合的蛋白胨大鼠小肠刷状缘膜（BBM）。本研究的目的是寻找新的食欲抑制肽。检测了来自鸡、猪、牛、牛肝和鸡蛋白色的蛋白胨与大鼠BBM结合、肠内分泌细胞系STC-1释放CCK和诱导大鼠饱腹感的活性。鸡和猪肉蛋白胨（ChickIP和PorkIP）结合BBM的能力最高，如用表面等离子体生物传感器所评估的。PorkP和ChickP从STC-1细胞释放CCK的量高于BconP，呈剂量依赖性，其中PorkP的刺激最高。PorkP而不是ChickP的口胃预负荷抑制食物摄入，与BconP相似，呈剂量依赖性。这些结果表明，PorkP直接与小肠CCK细胞相互作用，释放CCK，并抑制大鼠的食欲。
We found that soybean beta-conglycinin peptone (BconP) suppresses food intake through cholecystokinin (CCK) release from enteroendocrine cells in association with binding of the peptone to rat small intestinal brush border membrane (BBM). The aim of the present study was to find new appetite suppressing peptides. Peptones from chicken, pork, beef, beef liver, and egg white were examined for activities to bind with rat BBM, CCK-release from enteroendocrine cell line STC-1, and induce satiety in rats. Chicken and pork peptone (ChicklP and PorklP) bound to BBM with highest ability as evaluated with a surface plasmon biosensor. PorkP and ChickP released CCK in higher amounts than BconP from STC-1 cells dose-dependently, with highest stimulation by PorkP. An orogastric preload of PorkP but not ChickP, suppressed food intake similarly to BconP, dose-dependently. These results suggest that PorkP interacts directly with the small intestinal CCK cells to release CCK, and that it suppresses appetite in rats.