A part of cholinergic fibers in mouse superior cervical ganglia contain GABA or glutamate
A part of cholinergic fibers in mouse superior cervical ganglia contain GABA or glutamate
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DOI：
10.1016/j.brainres.2005.04.018
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发表时间：
2005-06-07
期刊：
影响因子：
2.9
通讯作者：
Nojyo, Y
中科院分区：
文献类型：
--
作者：
Ito, T;Iino, S;Nojyo, Y
谷氨酸和GABA是中枢神经系统中的主要氨基酸类神经递质，其在周围神经系统中的定位和功能尚不清楚。我们分别用囊泡乙酰胆碱转运体（VAchT）、GAD 65、囊泡谷氨酸转运体1-3（VGluTs 1 -3）、交感节前神经元（SPN）标记物、GABA能和谷氨酸能终末的抗体对小鼠上级颈神经节进行化学双染。所有GAD 65阳性终末均呈VAchT免疫反应，表明GABA能纤维起源于SPN。VGluT 2阳性终末与VAchT共定位，而VGluT 1和VGluT 3阳性终末未与VAchT共定位。所有VGluT 2免疫阳性终末均呈神经元型一氧化氮合酶（nNOS）免疫阳性，而约一半GABA免疫阳性纤维呈nNOS免疫阳性。讨论了这些纤维的来源。(c)2005 Elsevier B. V.保留所有权利。
The localizations and functions of glutamate and GABA, the major amino acid neurotransmitters in the central nervous system, are still unclear in the peripheral nervous system. We immunohistochemically double-stained mouse superior cervical ganglia with antibodies for the vesicular acetylcholine transporter (VAchT), GAD65, the vesicular glutamate transporters 1-3 (VGluTs1-3), the marker of the sympathetic preganglionic neuron (SPN), GABAergic, and glutamatergic terminals, respectively. All GAD65-positive terminals showed VAchT immunoreactivity, indicating that GABAergic fibers originate from SPNs. VGIuT2-immunoreactive terminals showing colocalization with VAchT were observed, but VGluT1 and 3 immunoreactive terminals were not. Colocalization of GAD65 and VGluT2 was rarely found. All VGIuT2-immunopositive terminals were also immunopositive for neuronal nitric oxide synthase (nNOS), a marker for the subpopulation of the SPNs, while about half of the GABA-immunopositive fibers were immunopositive for nNOS. The origin of these fibers was discussed. (c) 2005 Elsevier B.V. All rights reserved.