Fabry-Perot fiber-optic immunosensor based on suspended layer-by-layer (chitosan/polystyrene sulfonate) membrane
Fabry-Perot fiber-optic immunosensor based on suspended layer-by-layer (chitosan/polystyrene sulfonate) membrane
复制标题
DOI：
10.1016/j.snb.2013.06.093
复制
发表时间：
2013-11-01
影响因子：
8.4
通讯作者：
Leong, K. C.
中科院分区：
文献类型：
--
作者：
Chen, L. H.;Chan, C. C.;Leong, K. C.
提出了一种无标记光纤法布里-珀罗涉型(FPI)免疫传感器，该传感器是将壳聚糖和聚苯乙烯磺酸盐膜逐层自组装在中空纤维末端。将免疫球蛋白G固定在聚电解质底物上，通过测量底物的有效光学厚度来监测靶向抗免疫球蛋白G的检测。用相关和非相关的抗-Ig G/Ig G对研究该传感器的敏感性和特异性。该传感器对非相关抗体的灵敏度为0.033微米/(pg/mm(2))，检测下限为0.005 nm。实验结果证明了该传感器用于免疫传感的可行性。(C)2013爱思唯尔B.V.保留所有权利。
A label-free fiber-optic Fabry-Perot interferometric (FPI) immunosensor is proposed by layer-by-layer (LBL) self-assembly of a suspended chitosan and polystyrene sulfonate membrane at the tip of a hollow fiber. Immunoglobulin G (IgG) was immobilized on the polyelectrolyte substrate and the detection of target anti-immunoglobulin G (anti-IgG) was monitored through the measurement of substrate's effective optical thickness. The sensitivity and specificity of the sensor were studied using correlated and non-correlated anti-IgG/IgG pair. Sensitivity of 0.033 mu m/(pg/mm(2)) and limit of detection of 0.005 nM was achieved with the sensor displaying minimal response toward non-correlated anti-IgG. The results demonstrate the feasibility of the sensor for immunosensing. (C) 2013 Elsevier B.V. All rights reserved.