Structure Analysis of Carboxymethyl Starch by Capillary Electrophoresis and Enzymic Degradation

Structure Analysis of Carboxymethyl Starch by Capillary Electrophoresis and Enzymic Degradation
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毛细管电泳和酶降解法分析羧甲基淀粉的结构

DOI：
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发表时间：
2004
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通讯作者：
P. Mischnick
P. Mischnick
中科院分区：
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文献类型：
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作者：
W. Tüting;G. Albrecht;B. Volkert;P. Mischnick

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采用毛细管电泳法对取代度为1.2%~1.5%的羧甲基淀粉(CMS)在硼酸盐缓冲液中水解还原胺化后进行分析。将测定的单体组成与斯普林和鲁本的统计模型计算的数据进行了比较。此外，还用α-淀粉酶和淀粉糖苷酶对淀粉衍生物进行了彻底降解，并测定了葡萄糖的释放量。讨论了羧甲基淀粉的衍生化条件和性质。
Carboxymethyl starches (CMS) with a degree of substitution (DS) in the range of 1.2 to 1.5 were analysed by capillary electrophoresis (CE) after hydrolysis and reductive amination in a borate buffer. The monomer composition determined was compared to data calculated by the statistical models of Spurlin and Reuben. In addi­tion, the starch derivatives were exhaustively degraded by α-amylase and amylo­glucosidase and the amount of glucose liberated was determined. Results were discussed with regard to derivatisation conditions and properties of the carboxymethyl starches.