Two-compartment method for determination of the oxygen transfer rate with electrochemical sensors based on sulfite oxidation
Two-compartment method for determination of the oxygen transfer rate with electrochemical sensors based on sulfite oxidation
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DOI：
10.1002/biot.201100281
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发表时间：
2011-08-01
影响因子：
4.7
通讯作者：
Junne, Stefan
中科院分区：
文献类型：
--
作者：
Glazyrina, Julia;Materne, Eva;Junne, Stefan
由于氧气在水中的溶解度较低，溶解氧浓度是好氧生物过程中的一个重要参数。本文介绍了一种基于亚硫酸钠方法和电化学氧传感器相结合的新方法来测定摇瓶培养系统的氧转移速率(OTR)。该方法通过在线检测反应终点，取代了费力地滴定剩余亚硫酸盐。该方法分两步进行，可应用于不允许插入电极的任意烧瓶中。因此，该方法不依赖于在其中检测到OTR的容器类型。这一概念通过对标准有挡板的1-L摇瓶和不透明的超高产(TM)烧瓶的OTR值的测定来证明。在典型的摇动条件下，标准折流瓶的k(L)a值在220h(-1)达到值，而超产瓶的k(L)a值显著高于标准折流瓶(422h(-1))。
The dissolved oxygen concentration is a crucial parameter in aerobic bioprocesses due to the low solubility of oxygen in water. The present study describes a new method for determining the oxygen transfer rate (OTR) in shaken-culture systems based on the sodium sulfite method in combination with an electrochemical oxygen sensor. The method replaces the laborious titration of the remaining sulfite by an on-line detection of the end point of the reaction. This method is a two-step procedure that can be applied in arbitrary flasks that do not allow the insertion of electrodes. The method does not therefore depend on the type of vessel in which the OTR is detected. The concept is demonstrated by determination of the OTR for standard baffled 1-L shake flasks and for opaque Ultra Yield (TM) flasks. Under typical shaking conditions, k(L)a values in the standard baffled flasks reached values up to 220 h(-1), whereas the k(L)a values of the Ultra Yield flasks were significantly higher (up to 422 h(-1)).