Mouse strain-dependent variation in the course and outcome of chlamydial genital tract infection is associated with differences in host response
Mouse strain-dependent variation in the course and outcome of chlamydial genital tract infection is associated with differences in host response
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DOI：
10.1128/iai.65.8.3065-3073.1997
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发表时间：
1997-08-01
影响因子：
3.1
通讯作者：
Rank, RG
中科院分区：
文献类型：
--
作者：
Darville, T;Andrews, CW;Rank, RG
衣原体感染是否具有致病性或保护性结果可能由宿主反应来定义，我们用人类沙眼衣原体生物变种 E 血清型感染了 C57BL/6 (C57) 和 C3H/HeN (C3H) 小鼠，并且在选定的实验中，用沙眼衣原体 (MoPn) 小鼠肺炎病原体感染，我们比较了感染过程、组织病理学和宿主反应在 C3H 品系小鼠中，任一衣原体生物变型的感染持续时间显着增加，在感染 E 血清型小鼠中检查感染强度，在 C3H 品系中显着增加，组织病理学显示 C3H 小鼠中严重输卵管积水的发生率显着高于 C57 小鼠，相反，在所有感染的 C57 小鼠中观察到严重的子宫角扩张，而没有观察到C3H 小鼠感染了 E 血清型，而只有 25% 的小鼠感染了 MoPn。与 C3H 小鼠相比，C57 小鼠子宫角的急性炎症显着增加，抗原特异性反应的检查显示两种品系中的反应性质相似，γ 干扰素与白细胞介素 4 产生细胞的测定显示两种品系中 Th1 反应为主，免疫球蛋白 G1 (IgG1) 和 IgG2a 的血清酶联免疫吸附测定显示 IgG2a 为主两种品系中均存在抗体，尽管 C3H 小鼠中的抗体水平显着较高，淋巴细胞增殖研究显示，感染后 1 至 3 周，两种品系的髂淋巴结增殖增加。由于在 C57 品系中观察到感染较早根除，我们探讨了两种品系中肿瘤坏死因子 α (TNF-α) 的相对产生，与 C3H 小鼠相比，C57 小鼠生殖道分泌物中的 TNF-α 水平在感染期间显着增加。在感染的第一周，TNF-α的增加可能对宿主有益，因为可以更早地根除感染，从而预防输卵管感染，从而预防与生殖道衣原体感染相关的主要疾病后遗症。
Whether there is a pathogenic or protective outcome to chlamydial infection may be defined by the host response, We infected C57BL/6 (C57) and C3H/HeN (C3H) mice with the human biovar of Chlamydia trachomatis, serovar E, and, in select experiments, with the mouse pneumonitis agent of C, trachomatis (MoPn), We compared the courses of infection, histopathology, and host responses that resulted from these infections, The duration of infection with either chlamydial biovar was significantly increased in the C3H strain of mice, The intensity of infection was examined in mice infected with serovar E, and it was significantly increased in the C3H strain, Histopathology revealed the incidence of severe hydrosalpinx to be significantly greater in C3H mice than in C57 mice, In contrast, severe distention of the uterine horns was observed in all infected C57 mice compared to none of the C3H mice infected with serovar E and only 25% of those infected with MoPn. Acute inflammation was significantly increased in the uterine horns of C57 mice compared to that of C3H mice, Examination of antigen-specific responses revealed qualitatively similar responses in the two strains, Determination of gamma interferon- versus interleukin 4- producing cells revealed the predominance of a Th1 response in both strains, Serum enzyme-linked immunosorbent assays for immunoglobulin G1 (IgG1) and IgG2a revealed a predominance of IgG2a antibody in both strains, although the levels of antibody were significantly greater in C3H mice, Lymphocyte proliferation studies revealed increased proliferation in the iliac nodes of both strains at 1 to 3 weeks after infection, Because of the early eradication of infection observed in the C57 strain, we explored the relative production of tumor necrosis factor alpha (TNF-alpha) in the two strains, TNF-alpha levels were significantly increased in the genital tract secretions of C57 mice compared to that of C3H mice during the first week of infection, Increased TNF-alpha may be beneficial to the host by leading to earlier eradication of infection, thereby preventing infection of the oviduct and thus the major disease sequelae associated with chlamydial infection of the genital tract.