Identification of Nore1 as a potential Ras effector
Identification of Nore1 as a potential Ras effector
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DOI：
10.1074/jbc.273.10.5439
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发表时间：
1998-03-06
影响因子：
4.8
通讯作者：
Zhang, XF
中科院分区：
文献类型：
--
作者：
Vavvas, D;Li, X;Zhang, XF
小GTP结合蛋白Ras在细胞表面受体下游传递生长和分化信号中起关键作用。许多观察表明Ras通过与哺乳动物细胞中不同效应物的直接相互作用将细胞表面受体的信号传递到多个途径。我们已经确定了一种新的潜在Ras效应物或靶点，命名为Nore 1。Nore 1与已知的哺乳动物蛋白没有显著的序列相似性，并且缺乏可识别的催化结构域，但包含预测DAG_PE结合和SH 3结构域结合的序列基序。我们表明，Nore 1直接相互作用与Ras在体外的GTP依赖性的方式，和相互作用需要一个完整的Ras效应域。在COS-7和KB细胞中表皮生长因子受体激活后，Nore 1与Ras原位结合。
The small GTP-binding protein Ras is pivotal in transmitting growth and differentiation signals downstream of cell surface receptors. Many observations have indicated that Ras transmits signals from cell surface receptors into multiple pathways via direct interaction with different effecters in mammalian cells. We have identified a novel potential Ras effector or target named Nore1. Nore1 has no significant sequence similarity to known mammalian proteins and lacks an identifiable catalytic domain, but contains sequence motifs that predict DAG_PE binding and SH3 domain binding. We show that Nore1 directly interacts with Ras in vitro in a GTP-dependent manner, and the interaction requires an intact Ras effector domain. Nore1 becomes associated with Ras in situ following activation of epidermal growth factor receptor in COS-7 and in KB cells.