A crucial role for p80 TNF-R2 in amplifying p60 TNF-R1 apoptosis signals in T lymphocytes.
A crucial role for p80 TNF-R2 in amplifying p60 TNF-R1 apoptosis signals in T lymphocytes.
复制标题
p80 TNF-R2 在放大 T 淋巴细胞中 p60 TNF-R1 凋亡信号中发挥着至关重要的作用。
DOI：
10.1002/1521-4141(200002)30:2
复制
发表时间：
2000
影响因子：
5.4
通讯作者：
Lenardo,MJ
中科院分区：
文献类型：
--
作者：
Chan,FK;Lenardo,MJ
肿瘤坏死因子-α（TNF-α）可引起许多细胞反应，包括程序性细胞死亡或凋亡。TNF-α诱导的细胞凋亡在很大程度上归因于p60 TNF-R1受体。p80在TNF-α介导的细胞凋亡中的作用在很大程度上是未知的。我们现在提出的证据表明，信号通过p80开关在以前休眠的凋亡机制与p60。这种对p60相关凋亡的影响涉及半胱天冬酶的近端激活，并在存在强NF-κB诱导的情况下进行。我们评估了TRAF 2在p80辅助凋亡中的作用，发现TRAF 2蛋白的减少发生在p80而不是p60刺激下。然而，TRAF 2蛋白的减少可以在半胱天冬酶抑制剂的存在下与细胞凋亡分离。因此，p80 TNF-R2调节凋亡的一种方式是通过其放大来自p60 TNF-R1的内部凋亡信号转导的能力。
Tumor necrosis factor‐α (TNF‐α) can elicit many cellular responses including programmed cell death or apotosis. TNF‐α‐induced apoptosis has been largely attributed to the p60 TNF‐R1 receptor. The role of p80 in TNF‐α‐mediated apoptosis is largely unknown. We now present evidence that signaling through p80 switches on the previously dormant apoptotic machinery associated with p60. This effect on p60‐associated apoptosis involves the proximal activation of caspases and proceeds in the presence of strong NF‐κB induction. We evaluated the role of TRAF2 in p80‐assisted apoptosis and found that a decrease in TRAF2 protein occurs with p80 but not p60 stimulation. However, the decrease in TRAF2 protein can be dissociated from apoptosis in the presence of a caspase inhibitor. Hence, one means by which p80 TNF‐R2 regulates apoptosis is through its ability to amplify internally apoptotic signal transduction from p60 TNF‐R1.