IDENTIFICATION OF THE GENETIC-LOCUS FOR THE STRUCTURAL GENE AND A NEW REGULATORY GENE FOR THE SYNTHESIS OF REPRESSIBLE ALKALINE-PHOSPHATASE IN SACCHAROMYCES-CEREVISIAE
IDENTIFICATION OF THE GENETIC-LOCUS FOR THE STRUCTURAL GENE AND A NEW REGULATORY GENE FOR THE SYNTHESIS OF REPRESSIBLE ALKALINE-PHOSPHATASE IN SACCHAROMYCES-CEREVISIAE
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DOI：
10.1128/mcb.2.2.127
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发表时间：
1982-01-01
影响因子：
5.3
通讯作者：
OSHIMA, Y
中科院分区：
文献类型：
--
作者：
KANEKO, Y;TOHE, A;OSHIMA, Y
两条证据表明PHO 8基因编码S. cerevisiae：由温度敏感的pho 8突变体在允许温度（25 ℃）下产生的酶。C）的酶活比野生型菌株的酶活更不耐热，PHO 8基因对酶活的影响表现出基因剂量效应。pho 8基因座已被定位在染色体IV上，8厘摩远端rna 3。一个新的突变体携带pho 9基因被分离出来，它缺乏抑制性碱性磷酸酶，但具有正常表型的抑制性酸性磷酸酶的合成。pho 9基因分离独立于所有已知的pho调节基因，并没有显示基因剂量对抑制性碱性磷酸酶活性的影响。pho 9/pho 9二倍体几乎不产孢，接种在产孢培养基上时，没有表现出基因内重组的承诺。因此，pho 9突变体具有与pep 4突变体相似的表型，pep 4突变体是作为蛋白酶A和B以及羧肽酶Y水平降低的多效性突变体分离的。等位性试验表明pho 9和pep 4是等位的。
Two lines of evidence showed that the PHO8 gene encodes the structure of repressible, nonspecific alkaline phosphatase in S. cerevisiae: the enzyme produced by a temperature-sensitive pho8 mutant at the permissive temperature (25.degree. C) was more thermolabile than that of the wild-type strain, and the PHO8 gene showed a gene dosage effect on the enzyme activity. The pho8 locus has been mapped on chromosome IV, 8 centimorgans distal to rna3. A new mutant carrying the pho9 gene was isolated which lacks repressible alkaline phosphatase but has the normal phenotype for the synthesis of repressible acid phosphatase. The pho9 gene segregated independently of all known pho-regulatory genes and did not show the gene dosage effect on repressible alkaline phosphatase activity. The pho9/pho9 diploid hardly sporulated and showed no commitment to intragenic recombination when it was inoculated on sporulation medium. Hence the pho9 mutant has a phenotype similar to the pep4 mutant, which was isolated as a pleiotropic mutant with reduced levels of proteinases A and B and carboxypeptidase Y. An allelism test indicated that pho9 and pep4 are allelic.