Taking cell-matrix adhesions to the third dimension
Taking cell-matrix adhesions to the third dimension
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DOI：
10.1126/science.1064829
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发表时间：
2001-11-23
期刊：
影响因子：
56.9
通讯作者：
Yamada, KM
中科院分区：
文献类型：
--
作者：
Cukierman, E;Pankov, R;Yamada, KM
成纤维细胞和细胞外基质之间的粘附已经在体外进行了广泛的研究，但关于它们在体内的对应物还知之甚少。在这里，我们的特点是组成和功能的三维（3D）矩阵来自组织或细胞培养的粘连。“3D-基质粘连”与在2D基质上表征的局灶性和纤维性粘连的不同之处在于其α（5）β（1）和α（v）β（3）整联蛋白、桩蛋白、其他细胞骨架组分和局灶性粘连激酶（FAK）的酪氨酸磷酸化的含量。相对于2D底物，3D-基质相互作用也显示出增强的细胞生物学活性和缩小的整合素使用。这些独特的体内3D基质粘连在结构、定位和功能上与经典描述的体外粘连不同，因此它们可能与活生物体更具生物学相关性。
Adhesions between fibroblastic cells and extracellular matrix have been studied extensively in vitro, but tittle is known about their in vivo counterparts. Here, we characterized the composition and function of adhesions in three-dimensional (3D) matrices derived from tissues or cell culture. "3D-matrix adhesions" differ from focal and fibrillar adhesions characterized on 2D substrates in their content of alpha (5)beta (1) and alpha (v)beta (3) integrins, paxillin, other cytoskeletal components, and tyrosine phosphorylation of focal adhesion kinase (FAK). Relative to 2D substrates, 3D-matrix interactions also display enhanced cell biological activities and narrowed integrin usage. These distinctive in vivo 3D-matrix adhesions differ in structure, localization, and function from classically described in vitro adhesions, and as such they may be more biologically relevant to living organisms.