Direct four-dimensional structural and functional imaging of cardiovascular dynamics in mouse embryos with 1.5 MHz optical coherence tomography.
Direct four-dimensional structural and functional imaging of cardiovascular dynamics in mouse embryos with 1.5 MHz optical coherence tomography.
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DOI：
10.1364/ol.40.004791
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发表时间：
2015-10-15
期刊：
影响因子：
3.6
通讯作者：
Larina IV
中科院分区：
文献类型：
--
作者：
Wang S;Singh M;Lopez AL 3rd;Wu C;Raghunathan R;Schill A;Li J;Larin KV;Larina IV
小鼠胚胎心血管动力学的高分辨率三维（3D）成像是研究哺乳动物先天性心脏缺陷的迫切需要。在这里，我们展示了直接四维（4D）成像的心血管结构和功能的活小鼠胚胎在~43 Hz的体积率使用光学相干断层扫描（OCT）系统与~1.5 MHz傅立叶域锁模扫描激光源。将超快OCT成像与活小鼠胚胎培养方案相结合，随着时间的推移直接连续采集胚胎的3D体积，用于心脏动力学分析，而无需应用任何同步算法。我们提出了基于4D结构数据的心壁运动的时间分辨测量，报告4D散斑方差和血管系统的多普勒成像，并随着时间的推移量化空间分辨血流速度。这些结果表明，超高速4D成像方法可能是有效的小鼠胚胎心血管表型的有用工具。
High-resolution three-dimensional (3D) imaging of cardiovascular dynamics in mouse embryos is greatly desired to study mammalian congenital cardiac defects. Here, we demonstrate direct four-dimensional (4D) imaging of the cardiovascular structure and function in live mouse embryos at a ~43 Hz volume rate using an optical coherence tomography (OCT) system with a ~1.5 MHz Fourier domain mode-locking swept laser source. Combining ultrafast OCT imaging with live mouse embryo culture protocols, 3D volumes of the embryo are directly and continuously acquired over time for a cardiodynamics analysis without the application of any synchronization algorithms. We present the time-resolved measurements of the heart wall motion based on the 4D structural data, report 4D speckle variance and Doppler imaging of the vascular system, and quantify spatially resolved blood flow velocity over time. These results indicate that the ultra-high-speed 4D imaging approach could be a useful tool for efficient cardiovascular phenotyping of mouse embryos.