Biological Differences Between Ovarian Cancer-associated Fibroblasts and Contralateral Normal Ovary-derived Mesenchymal Stem Cells
Biological Differences Between Ovarian Cancer-associated Fibroblasts and Contralateral Normal Ovary-derived Mesenchymal Stem Cells
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DOI：
10.21873/anticanres.15649
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发表时间：
2022-03
影响因子：
2
通讯作者：
K. Ohashi;Tao-Sheng Li;S. Miura;Y. Hasegawa;K. Miura
中科院分区：
文献类型：
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作者：
K. Ohashi;Tao-Sheng Li;S. Miura;Y. Hasegawa;K. Miura
背景/目的：本研究旨在阐明卵巢癌相关成纤维细胞(OCA-CAF)与正常卵巢间充质干细胞(NO-MSCs)的生物学差异。材料和方法：将手术切除的卵巢癌组织和对侧正常卵巢组织标本切成小块培养，作为“外植体”。直接比较OCA-CAF和NO-MSCs的体外生长细胞数、增殖动力学、细胞表面标志物的表达水平以及3种miRNAs的差异。还研究了两组之间的差异表达基因。结果：两组均可获得数量相当的体外培养细胞。α-平滑肌肌动蛋白和miR-142在OCA-CAF中的表达显著增加，在体外细胞扩增过程中显著降低。两组间共鉴定出21个差异表达基因。结论：与非MSCs相比，OCA-CAF具有不同的生物学特性，可能在卵巢癌的发病机制中起重要作用。
Background/Aim: The aim of this study was to clarify the biological differences between ovarian cancer-associated fibroblasts (OCa-CAFs) and normal ovary-derived mesenchymal stem cells (NO-MSCs). Materials and Methods: Surgically resected ovarian cancer and contralateral normal ovarian tissue samples were cut into small pieces for culture as “explants”. The number of outgrown cells, their proliferative kinetics, and expression levels of cell surface markers of CAFs, as well as three miRNAs in OCa-CAFs and NO-MSCs were compared directly. Differentially expressed genes between both groups were also investigated. Results: Comparable numbers of outgrown cells were harvested from both groups. Significantly higher expression of α-smooth muscle actin and miR-142 was found in OCa-CAFs, which decreased significantly during ex vivo cell expansion. A total of 21 differentially expressed genes were identified between both groups. Conclusion: OCa-CAFs showed different biological properties in direct comparison with NO-MSCs, which might play major roles in the pathogenesis of ovarian cancer.