A Pilot Study of Urinary Exosomes in Alzheimer's Disease
A Pilot Study of Urinary Exosomes in Alzheimer's Disease
复制标题
尿液外泌体在阿尔茨海默病中的初步研究
DOI：
10.1159/000505851
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发表时间：
2020-06-01
影响因子：
3
通讯作者：
Zhang, Jiewen
中科院分区：
文献类型：
--
作者：
Sun, Ruihua;Wang, Huayuan;Zhang, Jiewen
背景：Exosome是由大多数细胞类型分泌的纳米级细胞外小泡，大量存在于体液中，包括血液、唾液、尿液、脑脊液和母乳。目的：探讨阿尔茨海默病(AD)患者尿外切体的形态、数量和病理蛋白水平的变化。方法：采用双抗体夹心酶联免疫吸附试验检测AD患者和正常对照组尿外切体中Aβ1-42和P-S396-tau(以CD63归一化)的水平。结果：AD患者尿外切体中Aβ1-42和P-S396-tau的表达水平均高于正常对照组。结论：AD患者尿外切体和Aβ1-42、P-S396-tau水平及尿外切体数量的差异可能为AD的早期诊断提供依据。
Background:Exosomes are nano-sized extracellular vesicles secreted by most cell types and abundantly present in body fluids, including blood, saliva, urine, cerebrospinal fluid, and breast milk. Exosomes can spread toxic amyloid-beta (A beta) and hyperphosphorylated tau between cells, contributing to neuronal loss in Alzheimer's disease (AD).Objective:To explore changes in the morphology, number, and pathological protein levels of urinary exosomes in AD patients compared with age-matched healthy subjects.Methods:In this study, enzyme-linked immunosorbent assay was used to detect the levels of A beta 1-42 and P-S396-tau (normalized by CD63) in urinary exosomes of AD patients and matched healthy subjects. We used transmission electron microscopy and nanoparticle tracking analysis to observe the exosomes.Results:We found that the levels of A beta 1-42 and P-S396-tau in the urinary exosomes of AD patients were higher than those of matched healthy controls. Exosomes taken from AD patients were more numerous.Conclusion:The differences in levels of A beta 1-42 and P-S396-tau and the quantity of urinary exosomes between AD patients and healthy controls may provide a basis for early diagnosis of AD.