Genomic analysis of bacteriophage PBECO4 infecting Escherichia coli O157:H7
Genomic analysis of bacteriophage PBECO4 infecting Escherichia coli O157:H7
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DOI：
10.1007/s00705-013-1718-3
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发表时间：
2013-11-01
影响因子：
2.7
通讯作者：
Myung, Heejoon
中科院分区：
文献类型：
--
作者：
Kim, Min Soo;Hong, Sung Sik;Myung, Heejoon
大肠杆菌O157:H7是一种人类病原体。我们从污水处理设施中分离出一种感染这种细菌的新型噬菌体。噬菌体PBECO4属于肌病毒科，具有等距头和可收缩尾。其DNA基因组长度为348113个碱基对，GC含量为34.09%。全基因组测序结果显示，PBECO4与肠杆菌噬菌体vB_KleM_RaK2和克罗纳杆菌噬菌体vB_CsaM_GAP32亲缘关系较远，相似度为10%，相似度为6%。共发现551个推定开放阅读框（orf）和6个tRNA基因。8个orf与编码结构蛋白的基因有关，9个与DNA包装有关，2个与DNA裂解活性有关，42个与复制和调控有关。490个orf没有进行功能注释。
Escherichia coli O157:H7 is a human pathogen. We isolated a novel bacteriophage infecting this bacterium from a sewage water treatment facility. Phage PBECO4 belongs to the family Myoviridae, having an isometric head and a contractile tail. It has a linear double-stranded DNA genome of 348,113 base pairs in length with a GC content of 34.09 %. Whole-genome sequencing revealed that PBECO4 is distantly related to enterobacteria phage vB_KleM_RaK2, with 10 % similarity, and Cronobacter phage vB_CsaM_GAP32 with 6 % similarity. Five hundred fifty-one putative open reading frames (ORFs) and six tRNA genes were found. Eight ORFs are related to genes encoding structural proteins, nine to DNA packaging, two to DNA lysis activity, and 42 to replication and regulation. Four hundred ninety ORFs have not been functionally annotated.