Electrophoretic Mobility of Double-Stranded DNA in Polymer Solutions and Gels with Tuned Structures
Electrophoretic Mobility of Double-Stranded DNA in Polymer Solutions and Gels with Tuned Structures
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DOI：
10.1021/ma500661r
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发表时间：
2014-05
期刊：
影响因子：
5.5
通讯作者：
Xiang Li;Kateryna Khairulina;U. Chung;T. Sakai
中科院分区：
文献类型：
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作者：
Xiang Li;Kateryna Khairulina;U. Chung;T. Sakai
我们报告了一项关于双链 DNA (dsDNA) 在具有精确控制网络结构的聚合物网络中迁移行为的系统实验研究。电泳迁移率 (μ) 似乎是碱基对数量 (n) 的幂律函数，μ ∼ n–γ，其中 0.36 < γ < 1.46。 γ 的方差通常使用具有约束释放的爬行模型或使用熵捕获 (ET) 模型来解释。然而，我们的结果表明，μ 值表示为幂律函数和 n 指数函数的乘积，这与任何现有模型都不同。幂律函数项对应于现有模型、劳斯模型或爬行模型。在聚合物凝胶中，我们观察到随着 n 的增加从劳斯模型到蠕动模型的交叉，而聚合物溶液中的迁移行为始终遵循劳斯模型。这些结果表明，指数函数项适应了 γ 的连续变化。
We report a systematic experimental study on the migration behavior of double-stranded DNA (dsDNA) in polymer networks with precisely controlled network structures. The electrophoretic mobility (μ) appeared to be a power law function of the number of base pairs (n), μ ∼ n–γ, with 0.36 < γ < 1.46. The variance in γ has been commonly explained using the reptation model with constraint release or using the entropic trapping (ET) model. However, our results indicated that the μ values were expressed as products of a power law function and an exponential function of n, which differs from any of the existing models. The power law function terms corresponded to the existing models, the Rouse model or the reptation model. In polymer gels, we observed a crossover from the Rouse to the reptation model with an increase in n, while the migration behavior in polymer solutions always obeyed the Rouse model. These results revealed that the continuous change in γ was accommodated by the exponential function terms.