Setting the Stage for Notch: The Drosophila Su(H)-Hairless Repressor Complex.
Setting the Stage for Notch: The Drosophila Su(H)-Hairless Repressor Complex.
复制标题
DOI：
10.1371/journal.pbio.1002524
复制
发表时间：
2016-07
期刊：
影响因子：
9.8
通讯作者：
Oswald F
中科院分区：
文献类型：
--
作者：
Borggrefe T;Oswald F
Notch信号在整个发育过程中反复使用，以保持干细胞的潜力，或者在其他情况下允许分化。在果蝇中，Notch信号转导的中心转录因子是CBF-1/RBP-J，SU(H)，LAG-1(CSL)-SU(H)，它在转录激活和抑制之间起着分子开关的作用。SU(H)通过与辅抑制子无毛(H)形成复合体来抑制转录。SU(H)-阻遏物复合体不仅与Notch胞内域(NICD)竞争，而且还配置局部染色质景观。在这一期中，袁和他的同事确定了SU(H)/H络合物的结构，表明SU(H)中的一个主要构象变化解释了为什么NICD和H的结合是相互排斥的。这本初级读物审查了最近的研究，这些研究揭示了决定Notch途径转录因子CSL/SU(H)是激活还是抑制转录的结构重排。
Notch signaling is iteratively used throughout development to maintain stem cell potential or in other instances allow differentiation. The central transcription factor in Notch signaling is CBF-1/RBP-J, Su(H), Lag-1 (CSL)—Su(H) in Drosophila—which functions as a molecular switch between transcriptional activation and repression. Su(H) represses transcription by forming a complex with the corepressor Hairless (H). The Su(H)-repressor complex not only competes with the Notch intracellular domain (NICD) but also configures the local chromatin landscape. In this issue, Yuan and colleagues determined the structure of the Su(H)/H complex, showing that a major conformational change within Su(H) explains why the binding of NICD and H is mutually exclusive. This Primer examines recent research that reveals the structural rearrangements that determine whether the Notch pathway transcription factor CSL/Su(H) activates or represses transcription.